Anti-VEGF Receptor 2 抗体 (ab11939)
Key features and details
- Rabbit polyclonal to VEGF Receptor 2
- Suitable for: Sandwich ELISA, IP, ELISA, Neutralising, WB, Flow Cyt, IHC-Fr
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-VEGF Receptor 2 antibody
VEGF Receptor 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VEGF Receptor 2 -
由来種
Rabbit -
特異性
The polyclonal antibody will detect native and recombinant human VEGFR2/KDR in ELISA experiments and on the surface or solubilized from different human cell types. -
アプリケーション
適用あり: Sandwich ELISA, IP, ELISA, Neutralising, WB, Flow Cyt, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human VEGF Receptor 2. Highly pure recombinant human soluble extracellular domain of KDR (110 kDa) (D1-D7).
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ポジティブ・コントロール
- IHC: Human spleen Flow Cyt: HUVEC (Human umbilical vein endothelial cell line) whole cell lysate and HDLEC (Human dermal lymphatic endothelial cell line) whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
This antibody is affinity purified from rabbit sera. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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sELISA pair antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11939の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228).
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab42228 as Capture. |
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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Neutralising |
Use at an assay dependent concentration. PubMed: 17991870
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WB | (1) |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 151 kDa.
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Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-Fr |
Use at an assay dependent concentration.
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特記事項 |
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Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab42228 as Capture. |
IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
Neutralising
Use at an assay dependent concentration. PubMed: 17991870 |
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 151 kDa. |
Flow Cyt
Use a concentration of 1 - 5 µg/ml. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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機能
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
関連疾患
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3791 Human
- Entrez Gene: 16542 Mouse
- Entrez Gene: 25589 Rat
- Omim: 191306 Human
- SwissProt: P35968 Human
- SwissProt: P35918 Mouse
- SwissProt: O08775 Rat
- Unigene: 479756 Human
see all -
別名
- CD309 antibody
- CD309 antigen antibody
- EC 2.7.10.1 antibody
see all
画像
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Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.
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All lanes : Anti-VEGF Receptor 2 antibody (ab11939)
Lane 1 : recombinant human soluble VEGFR-2
Lane 2 : recombinant native VEGFR-2
Secondary
All lanes : anti-rabbit AP-conjugated antibody
Predicted band size: 151 kDaSamples were loaded in 10% SDS-PAGE under reducing conditions.
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Flow Cytometry analysis of primary human dermal lymphatic endothelial cells (HDLEC) labelling VEGF Receptor 2 with ab11939.
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Flow Cytometry analysis of primary human umbilical vein endothelial cells (HUVEC) labelling VEGF Receptor 2 with ab11939.
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Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (57)
ab11939 は 57 報の論文で使用されています。
- Chen J et al. Bone marrow stromal cell-derived exosomal circular RNA improves diabetic foot ulcer wound healing by activating the nuclear factor erythroid 2-related factor 2 pathway and inhibiting ferroptosis. Diabet Med 40:e15031 (2023). PubMed: 36537855
- Hu G et al. Exosomal circCOL1A1 promotes angiogenesis via recruiting EIF4A3 protein and activating Smad2/3 pathway in colorectal cancer. Mol Med 29:155 (2023). PubMed: 37940881
- Lu Y et al. Inhibition of FNDC1 suppresses gastric cancer progression by interfering with Gβγ-VEGFR2 complex formation. iScience 26:107534 (2023). PubMed: 37670789
- Lan J et al. Cord Blood Natural Killer Cells Inhibit Sepsis Caused by Feces-Induced Acute Peritonitis via Increasing Endothelium Integrity. Cell Transplant 31:9636897221090257 (2022). PubMed: 35438589
- Abedpoor N et al. Cross Brain-Gut Analysis Highlighted Hub Genes and LncRNA Networks Differentially Modified During Leucine Consumption and Endurance Exercise in Mice with Depression-Like Behaviors. Mol Neurobiol 59:4106-4123 (2022). PubMed: 35476290