AB11934

Anti-VEGF Receptor 1 抗体 [EWF]

Anti-VEGF Receptor 1 antibody [EWF]

KO Validated
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(2 Publications)

Mouse Monoclonal VEGF Receptor 1 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human, Recombinant full length protein - Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FLT1.

別名を表示する

FLT, FRT, VEGFR1, FLT1, Vascular endothelial growth factor receptor 1, VEGFR-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor, FLT-1

3 Images

Collaborator

Immunoprecipitation - Anti-VEGF Receptor 1 antibody [EWF] (AB11934)

Immunoprecipitation of VEGFR1 in A-375 cells. Lysates were prepared and immunoprecipitation was performed using 2 μg of ab11934 pre-coupled to Protein A beads. Samples were then washed and processed for western blot.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

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Collaborator

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (AB11934)

ab11934 was shown to react with VEGFR1 in wild-type A-375 cells in Western blot with loss of signal observed in a VEGFR1 knockout cell line. Wild-type A-375 and VEGFR1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab11934 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (ab11934) at 1/500 dilution

Lane 1:

Wild-type A-375 lysate at 20 µg

Lane 2:

VEGFR1 knock-out A-375 lysate at 20 µg

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Supplier Data

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (AB11934)

All lanes:

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (ab11934) at 10 µg/mL

All lanes:

Conditioned media from insect cells expressing recombinant human soluble VEGF Receptor 1

Predicted band size: 151 kDa

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

EWF

アイソタイプ

IgG1

キャリアフリー

Yes

交差種

Human, Mouse

アプリケーション

applications

免疫原

Recombinant Full Length Protein corresponding to Human FLT1.

P17948

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-10 µg/mL", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-10 µg/mL", "WB-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Lyophilized

再構成

Reconstitute in water

精製方法

Affinity purification Protein G

精製に関する特記事項

Purified from TCS.

バッファー組成

Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

VEGF Receptor 1 also known as FLT-1 is a protein that plays a critical role in the regulation of angiogenesis. Its mass is approximately 180 kDa. FLT-1 is a receptor tyrosine kinase expressed mainly on the surface of endothelial cells but also in monocytes and macrophages. The receptor binds vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) which initiates a series of signaling cascades important for vascular development and function.

Biological function summary

FLT-1 mediates important cellular responses such as cell migration and proliferation. Although it often functions in conjunction with other receptors like VEGFR-2 FLT-1 has unique binding affinities that modulate the availability of VEGF for other receptors. FLT-1 proteins as part of complex signaling networks actively contribute to maintaining vascular homeostasis and regulating blood vessel permeability.

Pathways

FLT-1 is an important component in the VEGF signaling pathway and also part of the PlGF/VEGF pathway. The receptor serves as a decoy to regulate the amount of VEGF interaction with VEGFR-2 influencing endothelial cell function and vascular permeability. By sequestering VEGF FLT-1 helps maintain the balance in this angiogenic signaling network interacting with proteins like VEGF PlGF and the anti-VEGF agents that target these pathways.

FLT-1 is implicated in cancer and diabetic retinopathy. Overexpression or dysregulation of FLT-1 can contribute to tumor angiogenesis which can enhance tumor growth and metastasis. In diabetic retinopathy abnormal FLT-1 activity affects retinal blood vessels leading to vision impairment. Similar interactions involve VEGFR-2 and other downstream effectors emphasizing the potential of FLT-1 as a therapeutic target for conditions associated with excessive angiogenesis and vascular dysfunction.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Acts as a positive regulator of postnatal retinal hyaloid vessel regression (By similarity). May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1 (PubMed : 16685275).. Isoform 1. Phosphorylates PLCG.. Isoform 2. May function as decoy receptor for VEGFA.. Isoform 3. May function as decoy receptor for VEGFA.. Isoform 4. May function as decoy receptor for VEGFA.. Isoform 7. Has a truncated kinase domain; it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion.

See full target information FLT1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Life sciences 276:119432 PubMed33794253

2021

Hypoxia activates SUMO-1-HIF-1α signaling pathway to upregulate pro-inflammatory cytokines and permeability in human tonsil epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Lin,Mingjing Wang,Zhen Xiao,Zhiyan Jiang

Scientific reports 8:17924 PubMed30560942

2018

A peptide mimicking the binding sites of VEGF-A and VEGF-B inhibits VEGFR-1/-2 driven angiogenesis, tumor growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Farzaneh Behelgardi,Saber Zahri,Farhad Mashayekhi,Kamran Mansouri,S Mohsen Asghari

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-VEGF Receptor 1 抗体 [EWF]

Anti-VEGF Receptor 1 antibody [EWF]

Immunoprecipitation - Anti-VEGF Receptor 1 antibody [EWF] (AB11934)

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (AB11934)

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (AB11934)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

再構成

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise