Anti-VEGF 165A 抗体 - BSA and Azide free (ab291246)
Key features and details
- Goat polyclonal to VEGF 165A - BSA and Azide free
- Suitable for: Sandwich ELISA, WB
- Isotype: IgG
製品の概要
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製品名
Anti-VEGF 165A antibody - BSA and Azide free
VEGF 165A 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to VEGF 165A - BSA and Azide free -
由来種
Goat -
アプリケーション
適用あり: Sandwich ELISA, WBmore details -
種交差性
交差が予測される動物種: Human
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免疫原
Recombinant fragment corresponding to Human VEGF 165A aa 1 to the C-terminus.
Database link: P15692-4 -
ポジティブ･コントロール
- WB: Recombinant human VEGF protein.
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile water to a concentration of 0.1-1.0mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: 100% PBS
Buffer lyophilized from -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified by affinity chromatography employing immobilized hVEGF matrix. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab291246の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
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| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 27 kDa.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. |
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 27 kDa. |
ターゲット情報
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機能
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
組織特異性
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
関連疾患
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
配列類似性
Belongs to the PDGF/VEGF growth factor family. -
細胞内局在
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
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参照データベース
- Entrez Gene: 7422 Human
- Omim: 192240 Human
- SwissProt: P15692 Human
- Unigene: 73793 Human
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別名
- glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
- MVCD1 antibody
see all
画像
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Western blot - Anti-VEGF 165A antibody - BSA and Azide free (ab291246)
To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane.
Gel run under non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human VEGF165, respectively.
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Sandwich ELISA - Anti-VEGF 165A antibody - BSA and Azide free (ab291246)To detect hVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab291246 is required. This antibody, in conjunction with ab271201 as a biotinylated detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab291246 は論文での使用が確認できていません。
