AB118872

Anti-VDAC2 抗体

Name: Anti-VDAC2 抗体 (ab118872) | Abcam
Brand: Abcam Limited
SKU: AB118872
Price: 90000 JPY
Availability: InStock

Anti-VDAC2 antibody - Mitochondrial Loading Control

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(3 Publications)

Goat Polyclonal VDAC2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Voltage-dependent anion-selective channel protein 2 aa 250 to C-terminus.

別名を表示する

Non-selective voltage-gated ion channel VDAC2, VDAC-2, hVDAC2, Outer mitochondrial membrane protein porin 2, VDAC2

2 Images

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody - Mitochondrial Loading Control (AB118872)

Immunohistochemical staining of ab118872 at 5 μg/ml staining VDAC2 in Paraffin-embedded Human heart tissue followed by biotinylated anti-Goat IgG secondary antibody, alkaline phosphatasestreptavidin and chromogen

Unknown

Western blot - Anti-VDAC2 antibody - Mitochondrial Loading Control (AB118872)

All lanes:

Western blot - Anti-VDAC2 antibody - Mitochondrial Loading Control (ab118872) at 0.3 µg/mL

All lanes:

Human testis lysate in RIPA buffer at 35 µg

Predicted band size: 32 kDa

true

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human Voltage-dependent anion-selective channel protein 2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P45880

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5-5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 98% Tris buffered saline, 0.5% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Voltage-dependent anion channel 2 (VDAC2) also known as porin 31HL or P2 mechanically functions as a channel facilitating the transport of anions and metabolites across the outer mitochondrial membrane. This channel approximately 32 kDa in size is most prominent in the mitochondria but can also be observed in other cellular locations. It is involved in regulating both the entry and exit of small ions and molecules which is essential for mitochondrial function and energy production.

Biological function summary

VDAC2 plays significant roles beyond mere ion transport. It interacts with proteins involved in apoptosis such as Bcl-2 family proteins. Although not part of a larger complex its influence on the structure and permeability of the outer mitochondrial membrane impacts several cellular processes. The regulation of apoptosis by VDAC2 is key for cellular homeostasis and response to stress signals highlighting its role in maintaining normal cellular functions.

Pathways

Several cellular processes include VDAC2's role in apoptosis and energy metabolism. VDAC2 interacts with the intrinsic pathway of apoptosis in close association with proteins like Bax and Bak where it influences the release of cytochrome c from mitochondria. Additionally VDAC2 has a role in ATP transport and metabolic flux integral to the bioenergetics pathways by working with adenine nucleotide translocase (ANT) as part of the exchange of ATP and ADP.

Mutations or dysregulation of VDAC2 connect to cancer and neurodegenerative diseases. Its interaction with the Bcl-2 family proteins implicates it in apoptosis resistance observed in various cancers. Likewise VDAC2 dysfunction has links to neurodegenerative diseases through its impact on mitochondrial health as these conditions often display an imbalance in apoptotic pathways and energy metabolism.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane (PubMed : 8420959). The channel adopts an open conformation at zero mV and a closed conformation at both positive and negative potentials (PubMed : 8420959). There are two populations of channels; the main that functions in a lower open-state conductance with lower ion selectivity, that switch, in a voltage-dependent manner, from the open to a low-conducting 'closed' state and the other that has a normal ion selectivity in the typical high conductance, 'open' state (PubMed : 8420959). Binds various lipids, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed : 31015432). Binding of ceramide promotes the mitochondrial outer membrane permeabilization (MOMP) apoptotic pathway (PubMed : 31015432). Associates with the translocase of the outer mitochondrial membrane (TOM) complex and PINK1 kinase at depolarized mitochondria, this interaction stabilizes PINK1 at the outer mitochondrial membrane and triggers downstream mitophagy by the recruitment of the E3 ubiquitin ligase PRKN (PubMed : 40080546).. Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.

See full target information Voltage-dependent anion-selective channel protein 2

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2416 PubMed40069152

2025

VDAC2 and Bak scarcity in liver mitochondria enables targeting hepatocarcinoma while sparing hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shamim Naghdi,Piyush Mishra,Soumya Sinha Roy,David Weaver,Ludivine Walter,Erika Davies,Anil Noronha Antony,Xuena Lin,Gisela Moehren,Mark A Feitelson,Christopher A Reed,Tullia Lindsten,Craig B Thompson,Hien T Dang,Jan B Hoek,Erik S Knudsen,György Hajnóczky

Biochimica et biophysica acta 1852:2213-24 PubMed26248058

2015

Age-related changes in the proteostasis network in the brain of the naked mole-rat: Implications promoting healthy longevity.

Applications

Unspecified application

Species

Unspecified reactive species

Judy C Triplett,Antonella Tramutola,Aaron Swomley,Jessime Kirk,Kelly Grimes,Kaitilyn Lewis,Miranda Orr,Karl Rodriguez,Jian Cai,Jon B Klein,Marzia Perluigi,Rochelle Buffenstein,D Allan Butterfield

The Journal of biological chemistry 289:29285-96 PubMed25187518

2014

Translocation of glycogen synthase kinase-3β (GSK-3β), a trigger of permeability transition, is kinase activity-dependent and mediated by interaction with voltage-dependent anion channel 2 (VDAC2).

Applications

Unspecified application

Species

Unspecified reactive species

Masaya Tanno,Atsushi Kuno,Satoko Ishikawa,Takayuki Miki,Hidemichi Kouzu,Toshiyuki Yano,Hiromichi Murase,Toshiyuki Tobisawa,Makoto Ogasawara,Yoshiyuki Horio,Tetsuji Miura

View all publications

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Abcam product promise

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Anti-VDAC2 抗体

Anti-VDAC2 antibody - Mitochondrial Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody - Mitochondrial Loading Control (AB118872)

Western blot - Anti-VDAC2 antibody - Mitochondrial Loading Control (AB118872)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Abcam product promise