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Anti-VDAC1/Porin 抗体 [16G9E6BC4] - Mitochondrial Loading Control (ab110326)

Reviews (2)Q&A (1)References (48)
Western blot - Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)

    Key features and details

    • Mouse monoclonal [16G9E6BC4] to VDAC1/Porin - Mitochondrial Loading Control
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control
      VDAC1/Porin 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [16G9E6BC4] to VDAC1/Porin - Mitochondrial Loading Control

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Saccharomyces cerevisiae

    • 免疫原

      Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

    • 特記事項

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

      Product was previously marketed under the MitoSciences sub-brand.

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab110326の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.
    特記事項
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.

    ターゲット情報

    • 機能

      Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

    • 組織特異性

      Heart, liver and skeletal muscle.

    • 配列類似性

      Belongs to the eukaryotic mitochondrial porin family.

    • ドメイン

      Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.

    • 細胞内局在

      Mitochondrion outer membrane. Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • N2441 antibody
        • OMP2 antibody
        • POR1 antibody
        • hVDAC1 antibody
        • MGC111064 antibody
        • Mitochondrial Porin antibody
        • Outer mitochondrial membrane protein porin 1 antibody
        • Plasmalemmal porin antibody
        • Porin 31HL antibody
        • Porin 31HM antibody
        • VDAC antibody
        • VDAC-1 antibody
        • Vdac1 antibody
        • VDAC1_HUMAN antibody
        • Voltage dependent anion channel 1 antibody
        • Voltage dependent anion selective channel protein 1 antibody
        • Voltage-dependent anion-selective channel protein 1 antibody
        • YNL055C antibody
        • YNL2441C antibody
        see all

      画像

      • Western blot - Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)
        Western blot - Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)
        Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326) at 1 µg/ml + Yeast spheroplast membrane fraction.

        Predicted band size: 30 kDa

      参考文献 (48)

      ab110326 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab110326 は 48 報の論文で使用されています。

      • Rahman MT  et al. An engineered variant of MECR reductase reveals indispensability of long-chain acyl-ACPs for mitochondrial respiration. Nat Commun 14:619 (2023). PubMed: 36739436
      • Maiti P & Fontanesi F Metabolic Labeling of Mitochondrial Translation Products in Whole Cells and Isolated Organelles. Methods Mol Biol 2661:193-215 (2023). PubMed: 37166639
      • Davis J  et al. A dynamic actin cytoskeleton is required to prevent constitutive VDAC-dependent MAPK signalling and aberrant lipid homeostasis. iScience 26:107539 (2023). PubMed: 37636069
      • Liu Y  et al. Isoquercitrin from Apocynum venetum L. Exerts Antiaging Effects on Yeasts via Stress Resistance Improvement and Mitophagy Induction through the Sch9/Rim15/Msn Signaling Pathway. Antioxidants (Basel) 12:N/A (2023). PubMed: 38001792
      • Jahn H  et al. Phospholipids are imported into mitochondria by VDAC, a dimeric beta barrel scramblase. Nat Commun 14:8115 (2023). PubMed: 38065946
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control

       Poor
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Candida albicans Cell lysate - other (Cytosolic and mitochondrial fractions)
      Gel Running Conditions
      Reduced Denaturing (4-12% precast gel)
      Loading amount
      15 µg
      Treatment
      Induced with 10 mM proline for 1 h
      Specification
      Cytosolic and mitochondrial fractions
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Fitz Gerald S Silao

      Verified customer

      投稿 Apr 26 2021

      Immunohistochemistry (Frozen sections) abreview for Anti-VDAC/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control

       Inconclusive
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Sample
      Mouse Tissue sections (Brain)
      Specification
      Brain
      Permeabilization
      Yes - 0.3% Triton X-100
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      PROF. Chichung Lie

      Verified customer

      投稿 Aug 01 2013

      Question

      Dear Technical,



      Good day! We have a customer who has placed an order for the above antibody but it is in back order and will not be available before March. Our customer is wondering if there is any alternative antibodies that they can use instead, as they need this before the middle of Feb.



      I was wondering if you could advise me about any alternative antibody that can be used.



      I hope to hear from you soon.



      Kind regards,

      Read More
      Answer

      Thank you for your enquiry.
      Currently, this antibody (ab34726) is out of stock. However, the good news is that we have some alternative products against the same target protein (VDAC/Porin). I do not know in which species/application your customer would like to use the other product so please advice her/him to read the products datasheets carefully to see which one would be suitable.
      - ab110326: https://www.abcam.com/vdac-porin-antibody-16g9e6bc4-ab110326.html
      - ab61273: https://www.abcam.com/vdac-porin-antibody-mitochondrial-marker-ab61273.html
      - ab96140: https://www.abcam.com/vdac-porin-antibody-16g9e6bc4-ab110326.html
      I hope this helps and if I can assist further, please do not hesitate to contact me.

      Read More

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