AB174279

Anti-VCAM1 抗体 [EPR5038(2)]

Anti-VCAM1 antibody [EPR5038(2)]

RabMAb
Recombinant
KO Validated
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(32 Publications)

Rabbit Recombinant Monoclonal VCAM1 antibody. Suitable for IP, WB and reacts with Mouse, Human samples. Cited in 32 publications.

別名を表示する

CD106, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1

5 Images

Supplier Data

Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

Immunoprecipitation. ab174279 at 1/1000 labeling VCAM1 immunoprecipitated from NIH3T3 cell lysate using ab174279 at 1/10.

All lanes:

Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

Predicted band size: 81 kDa

Observed band size: 100 kDa

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Lab

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

ab174279 was shown to react with VCAM1 in treated wild-type A549 cells in western blot. Loss of signal was observed when treated VCAM1 knockout cell line ab273758 (knockout cell lysate ab275504) was used. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab174279 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1 in 5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.

All lanes:

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 30 µg

Lane 2:

Wild-type A549 TNF-a treated (10 ng/mL, 16h) cell lysate at 30 µg

Lane 3:

VCAM1 knockout A549 cell lysate at 30 µg

Lanes 3 - 4:

Western blot - Human VCAM1 knockout A549 cell line (<a href='/products/cell-lines/human-vcam1-knockout-a549-cell-line-ab273758'>ab273758</a>)

Lane 4:

VCAM1 knockout A549 TNF-a treated (10 ng/mL, 16h) cell lysate at 30 µg

Predicted band size: 81 kDa

Observed band size: 105 kDa

false

Exposure time: 20s

Supplier Data

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

All lanes:

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

HuT-78 lysate at 10 µg

Lane 3:

NIH3T3 lysate at 10 µg

Predicted band size: 81 kDa

false

Unknown

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

Lane 1:

Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 20 µg

Lane 2:

SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate at 20 µg

Lane 3:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 4:

J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg

Lane 5:

LADMAC (Mouse bone marrow monocyte macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 81 kDa

Observed band size: 100 kDa

false

Exposure time: 15s

Unknown

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

Lane 1:

HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

HUVEC treated with 10 ng/ml TNF-α for 16 h, whole cell lysate at 20 µg

Lane 3:

bEND.3 (Mouse brain endothelioma) whole cell lysate at 20 µg

Lane 4:

bEND.3 treated with 10 μg/ml LPS for 24 h, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 81 kDa

Observed band size: 100 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5038(2)

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.

Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed : 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed : 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed : 22970700). Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed : 35210567).

See full target information VCAM1

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文献 (32)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1548660 PubMed40365315

2025

DC. Alleviates adenine-induced chronic kidney disease by regulating ferroptosis in C57BL/6 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jong Min Kim,Yiseul Kim,Hyun-Jin Na,Haeng Jeon Hur,Sang-Hee Lee,Mi Jeong Sung

Cardiovascular research 121:1108-1120 PubMed40171617

2025

Endothelial insulin-like growth factor-1 signalling regulates vascular barrier function and atherogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Drozd,Alexander-Francisco Bruns,Nadira Y Yuldasheva,Azhar Maqbool,Hema Viswambharan,Anna Skromna,Natallia Makava,Chew W Cheng,Piruthivi Sukumar,Lauren Eades,Andrew M N Walker,Kathryn J Griffin,Stacey Galloway,Nicole T Watt,Natalie Haywood,Victoria Palin,Nele Warmke,Helen Imrie,Katherine Bridge,David J Beech,Stephen B Wheatcroft,Mark T Kearney,Richard M Cubbon

Scientific reports 14:16766 PubMed39034310

2024

Refining the optimal CAF cluster marker for predicting TME-dependent survival expectancy and treatment benefits in NSCLC patients.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Li,Rui Wang,Guo-Wei Liu,Zi-Yang Peng,Ji-Chang Wang,Guo-Dong Xiao,Shou-Ching Tang,Ning Du,Jia Zhang,Jing Zhang,Hong Ren,Xin Sun,Yi-Ping Yang,Da-Peng Liu

Pharmacology 109:169-179 PubMed38583431

2024

Fisetin Suppresses Atherosclerosis by Inhibiting Ferroptosis-Related Oxidative Stress in Apolipoprotein E Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiufang Jiang,Yanling Lei,Yajuan Yin,Fangfang Ma,Mingqi Zheng,Gang Liu

Blood 142:1918-1927 PubMed37774369

2023

Cold exposure induces vaso-occlusion and pain in sickle mice that depend on complement activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zalaya K Ivy,John D Belcher,Iryna A Khasabova,Chunsheng Chen,Joseph P Juliette,Fuad Abdulla,Conglin Ruan,Kaje Allen,Julia Nguyen,Victoria M Rogness,Joan D Beckman,Sergey G Khasabov,Kalpna Gupta,Ronald P Taylor,Donald A Simone,Gregory M Vercellotti

International journal of genomics 2023:8437898 PubMed37056786

2023

Inhibition of lncRNA NFIA-AS1 Alleviates Abnormal Proliferation and Inflammation of Vascular Smooth Muscle Cells in Atherosclerosis by Regulating miR-125a-3p/AKT1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhu,Xiaofeng Tian,Yan Wang,Chengxiang Wang,Naiquan Yang,Lianghong Ying,Hongyan Niu

Redox biology 59:102587 PubMed36608590

2023

PM2.5 increases susceptibility to acute exacerbation of COPD via NOX4/Nrf2 redox imbalance-mediated mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoye Fan,Tingting Dong,Kun Yan,Xinxin Ci,Liping Peng

Experimental and therapeutic medicine 24:737 PubMed36478888

2022

KLF2 alleviates endothelial cell injury and inhibits the formation of THP‑1 macrophage‑derived foam cells by activating Nrf2 and enhancing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Tan,Hongqiang Ren,Yijun Liu,Hanxuan Yang,Qian Luo,Xuejun Deng

Frontiers in pharmacology 13:941400 PubMed36120369

2022

Deciphering the combination mechanisms of Gualou-Xiebai herb pair against atherosclerosis by network pharmacology and HPLC-Q-TOF-MS technology.

Applications

Unspecified application

Species

Unspecified reactive species

Yarong Liu,Hua Zhong,Pengbo Xu,An Zhou,Lidan Ding,Jingwen Qiu,Hongfei Wu,Min Dai

Journal of neuroinflammation 19:149 PubMed35705998

2022

SARS-CoV-2 productively infects human brain microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rui-Cheng Yang,Kun Huang,Hui-Peng Zhang,Liang Li,Yu-Fei Zhang,Chen Tan,Huan-Chun Chen,Mei-Lin Jin,Xiang-Ru Wang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VCAM1 抗体 [EPR5038(2)]

Anti-VCAM1 antibody [EPR5038(2)]

Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (32)

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Abcam product promise