Anti-VAV1 抗体 (ab245440)
Key features and details
- Rabbit polyclonal to VAV1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-VAV1 antibody
VAV1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VAV1 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human VAV1 aa 175-225. The exact sequence is proprietary.
Database link: P15498 -
ポジティブ･コントロール
- WB: Jurkat whole cell lysate. IP: VAV1 IP in Jurkat whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245440 was affinity purified using an epitope specific to VAV1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245440の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
20 µl/mg lysate |
|
| WB |
1/1000.
|
| 特記事項 |
|---|
|
IP
Use at an assay dependent concentration. 20 µl/mg lysate |
|
WB
1/1000. |
ターゲット情報
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機能
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation. -
組織特異性
Widely expressed in hematopoietic cells but not in other cell types. -
配列類似性
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
ドメイン
The DH domain is involved in interaction with CCPG1. -
翻訳後修飾
Phosphorylated on tyrosine residues. - Information by UniProt
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参照データベース
- Entrez Gene: 7409 Human
- Omim: 164875 Human
- SwissProt: P15498 Human
- Unigene: 116237 Human
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別名
- Oncogene vav antibody
- p95Vav antibody
- Proto-oncogene vav antibody
see all
画像
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Western blot - Anti-VAV1 antibody (ab245440)All lanes : Anti-VAV1 antibody (ab245440) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Exposure time: 30 secondsPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-VAV1 antibody (ab245440)VAV1 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245440 used for IP at 20 µl per reaction. For WB at a 1/250 dilution.Lane 1: ab245440 IP in Jurkat whole cell lysate.
Lane 2: Control IgG in Jurkat whole cell lysate.
Chemiluminescence detection: 30 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245440 は論文での使用が確認できていません。