Anti-Vav proteins 抗体 [EP482Y] (ab40875)
![Western blot - Anti-Vav proteins antibody [EP482Y] (ab40875)](https://www.abcam.co.jp/ps/products/40/ab40875/Images/ab40875-54168-anti-vav-proteins-antibody-ep482y-western-blot.jpg "Western blot - Anti-Vav proteins antibody [EP482Y] (ab40875)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP482Y] to Vav proteins
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Vav proteins antibody [EP482Y]
Vav proteins 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP482Y] to Vav proteins -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Vav proteins aa 750-850 (C terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- Jurkat cells lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP482Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40875の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (2) |
1/2000. Predicted molecular weight: 98 kDa. Also 101 or 87 kDa, depending on the isoform.
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| 特記事項 |
|---|
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/2000. Predicted molecular weight: 98 kDa. Also 101 or 87 kDa, depending on the isoform. |
ターゲット情報
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関連性
The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signalling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response. -
細胞内局在
Cytoplasmic and Plasma membrane VAV1, Cytoplasm - VAV2 & VAV3 -
参照データベース
- Entrez Gene: 10451 Human
- Entrez Gene: 7409 Human
- Entrez Gene: 7410 Human
- Omim: 164875 Human
- Omim: 600428 Human
- Omim: 605541 Human
- SwissProt: P15498 Human
- SwissProt: P52735 Human
see all -
別名
- 2810040F13Rik antibody
- A530094I06Rik antibody
- AA986410 antibody
see all
画像
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Western blot - Anti-Vav proteins antibody [EP482Y] (ab40875)Anti-Vav proteins antibody [EP482Y] (ab40875) at 1/2000 dilution + Jurkat cell lysate at 10 µg
Predicted band size: 98 kDa
Observed band size: 98 kDa -
![Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] (ab40875)](https://www.abcam.co.jp/ps/products/40/ab40875/Images/ab40875-54167-anti-vav-proteins-antibody-ep482y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] (ab40875)Overlay histogram showing Jurkat cells stained with ab40875 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40875, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Anti-Vav proteins antibody [EP482Y] (ab40875)](https://www.abcam.co.jp/ps/products/40/ab40875/Images/ab40875-3-benefits-of-recombinant-antibodies.png)
Anti-Vav proteins antibody [EP482Y] (ab40875)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab40875 は 6 報の論文で使用されています。
- Bacchelli C et al. Mutations in linker for activation of T cells (LAT) lead to a novel form of severe combined immunodeficiency. J Allergy Clin Immunol 139:634-642.e5 (2017). PubMed: 27522155
- Gastaldello S et al. Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells. PLoS Pathog 9:e1003664 (2013). Human . PubMed: 24130483
- Pauker MH et al. Studying the dynamics of SLP-76, Nck, and Vav1 multimolecular complex formation in live human cells with triple-color FRET. Sci Signal 5:rs3 (2012). IP . PubMed: 22534133
- Zyss D et al. Casein kinase I delta controls centrosome positioning during T cell activation. J Cell Biol 195:781-97 (2011). ICC/IF . PubMed: 22123863
- Barda-Saad M et al. Cooperative interactions at the SLP-76 complex are critical for actin polymerization. EMBO J 29:2315-28 (2010). PubMed: 20562827
- Gianni D et al. The involvement of the tyrosine kinase c-Src in the regulation of reactive oxygen species generation mediated by NADPH oxidase-1. Mol Biol Cell 19:2984-94 (2008). WB ; Human . PubMed: 18463161