Anti-VASP (phospho S157) 抗体 (ab47268)
Key features and details
- Rabbit polyclonal to VASP (phospho S157)
- Suitable for: ELISA, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-VASP (phospho S157) antibody
VASP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VASP (phospho S157) -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human VASP aa 100-200 (phospho S157).
Database link: P50552 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorlyation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47268の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 42 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 42 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. -
組織特異性
Highly expressed in platelets. -
配列類似性
Belongs to the Ena/VASP family.
Contains 1 WH1 domain. -
ドメイン
The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
The WH1 domain mediates interaction with XIRP1. -
翻訳後修飾
Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239. -
細胞内局在
Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. - Information by UniProt
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参照データベース
- Entrez Gene: 7408 Human
- Entrez Gene: 22323 Mouse
- Entrez Gene: 361517 Rat
- Omim: 601703 Human
- SwissProt: P50552 Human
- SwissProt: P70460 Mouse
- Unigene: 515469 Human
- Unigene: 9684 Mouse
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別名
- Vasodilator stimulated phosphoprotein antibody
- Vasodilator-stimulated phosphoprotein antibody
- VASP antibody
- VASP_HUMAN antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S157) antibody (ab47268)ab47268, at 1/50 dilution, staining VASP in Human tonsil tissue by Immunohistochemistry, Paraffin-embedded tissue. Left panel: no peptide; right panel: with immunizing phosphopeptide.
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Western blot - Anti-VASP (phospho S157) antibody (ab47268)All lanes : Anti-VASP (phospho S157) antibody (ab47268)
Lane 1 : NIH/3T3 cell extract treated with forskolin (40µM, 30 min)
Lane 2 : NIH/3T3 cell extract
Predicted band size: 42 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab47268 は 2 報の論文で使用されています。
- Wang L et al. Asiaticoside produces an antidepressant-like effect in a chronic unpredictable mild stress model of depression in mice, involving reversion of inflammation and the PKA/pCREB/BDNF signaling pathway. Mol Med Rep 22:2364-2372 (2020). PubMed: 32705202
- Maass PG et al. PDE3A mutations cause autosomal dominant hypertension with brachydactyly. Nat Genet 47:647-53 (2015). PubMed: 25961942