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Anti-VASP (phospho S157) 抗体 [5C6] (ab58555)

Reviews (1)Q&A (1)References (7)
Flow Cytometry - Anti-VASP (phospho S157) antibody [5C6] (ab58555)

    Key features and details

    • Mouse monoclonal [5C6] to VASP (phospho S157)
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-VASP (phospho S157) antibody [5C6]
      VASP 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [5C6] to VASP (phospho S157)

    • 由来種

      Mouse

    • 特異性

      This antibody recognizes the the ~50 kDa VASP protein phosphorylated at Ser157. It does not cross-react with non-phosphorylated VASP or with un-related phosphorylation sites.

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide corresponding to Human VASP (phospho S157) conjugated to keyhole limpet haemocyanin.

    • ポジティブ･コントロール

      • Vanadate-treated A431 cells

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Preservative: 0.1% Sodium azide
    • Concentration information loading...

    • 精製度

      Size exclusion

    • 特記事項（精製）

      Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography.

    • ポリ/モノ

      モノクローナル

    • クローン名

      5C6

    • アイソタイプ

      IgG1

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab58555の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use 0.5µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    特記事項
    Flow Cyt
    Use 0.5µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    ターゲット情報

    • 機能

      Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

    • 組織特異性

      Highly expressed in platelets.

    • 配列類似性

      Belongs to the Ena/VASP family.
      Contains 1 WH1 domain.

    • ドメイン

      The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
      The WH1 domain mediates interaction with XIRP1.

    • 翻訳後修飾

      Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.

    • 細胞内局在

      Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Vasodilator stimulated phosphoprotein antibody
        • Vasodilator-stimulated phosphoprotein antibody
        • VASP antibody
        • VASP_HUMAN antibody

      画像

      • Flow Cytometry - Anti-VASP (phospho S157) antibody [5C6] (ab58555)
        Flow Cytometry - Anti-VASP (phospho S157) antibody [5C6] (ab58555)
        Overlay histogram showing THP1 cells stained with ab58555 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58555, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      参考文献 (7)

      ab58555 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab58555 は 7 報の論文で使用されています。

      • O'Banion CP  et al. Compartmentalized cAMP Generation by Engineered Photoactivated Adenylyl Cyclases. Cell Chem Biol 26:1393-1406.e7 (2019). PubMed: 31353320
      • O'Banion CP  et al. Design, construction, and validation of optogenetic proteins. Methods Enzymol 621:171-190 (2019). PubMed: 31128778
      • O'Banion CP  et al. Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase. Cell Chem Biol 25:100-109.e8 (2018). PubMed: 29104065
      • Li XF  et al. Downregulation of the long noncoding RNA MBNL1-AS1 protects sevoflurane-pretreated mice against ischemia-reperfusion injury by targeting KCNMA1. Exp Mol Med 50:115 (2018). PubMed: 30185781
      • Neukamm SS  et al. Phosphorylation of serine 1137/1138 of mouse insulin receptor substrate (IRS) 2 regulates cAMP-dependent binding to 14-3-3 proteins and IRS2 protein degradation. J Biol Chem 288:16403-15 (2013). Human . PubMed: 23615913
      • Mise N  et al. Zyxin is a transforming growth factor-ß (TGF-ß)/Smad3 target gene that regulates lung cancer cell motility via integrin a5ß1. J Biol Chem 287:31393-405 (2012). WB . PubMed: 22778267
      • Martin-Padura I  et al. Spontaneous cell fusion of acute leukemia cells and macrophages observed in cells with leukemic potential. Neoplasia 14:1057-66 (2012). PubMed: 23226099

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      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-VASP (phospho S157) antibody [5C6]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Heart)
      Gel Running Conditions
      Reduced Denaturing (10%)
      Loading amount
      20 µg
      Specification
      Heart
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Jun 16 2019

      Question

      Customer kindly contacted us as they had excessive antibody loss during buffer exchange and labeling. To help with their troubleshooting of this, they where hoping that we could explain the use of PEG and sucrose as buffer for this AB as compared with other stabilizers such as BSA. Is this product sticky so that during buffer exchange it may aggregate?

      Read More
      Answer

      Thank you for contacting us regarding ab58555.

      I have contacted the lab on your behalf, the antibody would be fine as well in a different buffer containing another stabilizing agent e.g. BSA. However this product is provided in PEG and sucrose as was found the antibody was suffering less during lyophilization.

      The antibody itself is not stickier than other antibodies. It seems possible that the buffer with the PEG and sucrose is responsible of the low yield that you are getting after the conjugation. The buffer exchange method may need to be adjusted for improved performance.

      We would recommend use of one of our concentration kits for performing buffer exchange with this product. Our antibody concentration and clean up kits, Ab102776 and ab102778utilizes a simple spin column to easily and quickly buffers. These should work in any buffer containing PEG and are covered by our Abpromise guarantee. Which guarantees scientific support, replacement or refund should any Abcam product not perform as indicated on the datasheet. More information about Ab102776, ab102778 and our Abpromise may be found at the link below.



      Ab102776:https://www.abcam.com/Antibody-Concentration-Kit-1-column-ab102776.html

      Ab102778:https://www.abcam.com/Antibody-Concentration-Kit-3-columns-ab102778.html

      https://www.abcam.com/index.html?pageconfig=abpromise

      I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

      Read More

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      For licensing inquiries, please contact partnerships@abcam.com