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AB109321

Anti-VASP 抗体 [EPR1337(2)]

Anti-VASP antibody [EPR1337(2)]

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(1 Publication)

Rabbit Recombinant Monoclonal VASP antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

Vasodilator-stimulated phosphoprotein, VASP

6 Images
Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)
  • WB

Unknown

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

All lanes:

Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/10000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human platelet lysate at 10 µg

Predicted band size: 39 kDa

Observed band size: 46 kDa

false

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)
  • WB

Lab

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/1000 dilution

All lanes:

HEK293 cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 39 kDa,85 kDa

Observed band size: 100 kDa,46 kDa

false

Immunoprecipitation - Anti-VASP antibody [EPR1337(2)] (AB109321)
  • IP

Lab

Immunoprecipitation - Anti-VASP antibody [EPR1337(2)] (AB109321)

ab109321 (purified) at 1/30 immunoprecipitating VASP in HEK293 cell lysate (Lane 1). Lane 2 - rabbit monoclonal IgG instead of ab109321 in HEK293 lysates. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-VASP antibody [EPR1337(2)] (ab109321)

Predicted band size: 39 kDa

Observed band size: 46 kDa

false

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)
  • WB

Lab

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 46 kDa

false

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)
  • WB

Unknown

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

Lanes 1-4 : Merged signal (red and green). Green - ab109321 observed at 46 kDa. Red - loading control ab8245 observed at 36 kDa.

ab109321 Anti-VASP antibody [EPR1337(2)] was shown to specifically react with VASP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265892 (knockout cell lysate ab257792) was used. Wild-type and VASP knockout samples were subjected to SDS-PAGE. ab109321 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/1000 dilution

Lane 1:

Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

VASP knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Western blot - Human VASP knockout HeLa cell line (<a href='/products/cell-lines/human-vasp-knockout-hela-cell-line-ab265892'>ab265892</a>)

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 39 kDa

Observed band size: 46 kDa

false

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)
  • WB

Lab

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/5000 dilution

All lanes:

THP-1 cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 39 kDa,60 kDa,61 kDa,79 kDa

Observed band size: 46 kDa,60 kDa,79 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-VASP antibody [EPR1337(2)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR1337(2)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/70", "IP-species-notes": "<p><strong>For unpurified use at 1/10 - 1/100.</strong></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/10000.</strong></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

VASP also known as Vasodilator-Stimulated Phosphoprotein is a 46-50 kDa protein involved in actin filament assembly and elongation. It plays an important role in cellular motility and the maintenance of cell shape. VASP is expressed in many cell types but is notably present in platelets and endothelial cells. It is an important player in signal transduction pathways that lead to cytoskeletal reorganization influencing cell movement and adhesion dynamics.
Biological function summary

VASP is an important component in cell movement regulation. It acts within a larger complex of actin-binding proteins that control the assembly of actin filaments. This protein influences cell adhesion migration and signal transduction processes. Through these actions VASP influences cellular responses to environmental cues making it essential for cellular dynamics and integrity during development and wound healing.

Pathways

VASP connects to significant signaling pathways such as the PI3K/Akt pathway and the cAMP pathway. It often interacts with proteins like c-Abl tyrosine kinase and Mena contributing to actin dynamics regulation. These pathways are critical for transducing signals from extracellular environments to intracellular responses allowing for coordinated cellular functions in processes such as cell migration and growth.

VASP plays a role in cardiovascular disease and cancer-related metastasis due to its function in cell motility and adhesion. Abnormal VASP activity is associated with impaired endothelial cell function and contributes to atherosclerosis development. Additionally it interacts with proteins like Mena and fascin in cancer cells aiding in metastatic progression by enhancing motility and invasive capabilities.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
See full target information VASP
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

ACS pharmacology & translational science 4:1567-1577 PubMed34661075

2021

Inhibition of Arginine Methylation Impairs Platelet Function.

Applications

Unspecified application

Species

Unspecified reactive species

Alistair James Marsden,David R J Riley,Antonia Barry,Jawad S Khalil,Barbara-Ann Guinn,Neil T Kemp,Francisco Rivero,Pedro Beltran-Alvarez
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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