AB181067

Anti-VAPA 抗体 [EPR13589(B)] - N-terminal

Name: Anti-VAPA 抗体 [EPR13589(B)] - N-terminal (ab181067) | Abcam
Brand: Abcam Limited
SKU: AB181067
Price: 46000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-VAPA antibody [EPR13589(B)] - N-terminal

RabMAb
Recombinant
詳細を見る

(2 Reviews)

(7 Publications)

Rabbit Recombinant Monoclonal VAPA antibody. N-terminal. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.

別名を表示する

VAP33, VAPA, Vesicle-associated membrane protein-associated protein A, VAMP-A, VAMP-associated protein A, VAP-A, 33 kDa VAMP-associated protein, VAP-33

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (AB181067)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (AB181067)

Immunohistochemical analysis of Human fetal colon tissue staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (AB181067)

Immunohistochemical analysis of Human ovarian carcinoma staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-VAPA antibody [EPR13589(B)] - N-terminal (AB181067)

All lanes:

Western blot - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067) at 1/20000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

HEK293 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR13589(B)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Vesicle-associated membrane protein-associated protein A (VAPA) also known as VAP-A is an integral membrane protein involved in intracellular membrane trafficking. It has a molecular weight of approximately 33 kDa and is expressed in multiple tissues including the brain liver and muscles. Mechanically VAPA functions in the process of vesicle docking and fusion at membranes which is essential for the trafficking of proteins and lipids within cells. This protein is also a part of the endoplasmic reticulum (ER) and contributes to the formation of membrane contact sites between the ER and other organelles.

Biological function summary

In the cellular environment VAPA plays an important role in maintaining homeostasis by regulating lipid transport and metabolism. It forms a complex with several other proteins such as OSBP (oxysterol-binding protein) and CERT (ceramide transport protein) which facilitates the exchange of lipids between the ER and the Golgi apparatus. This interaction underlines VAPA’s role in lipid droplet dynamics and the synthesis of phosphatidylinositols which are critical for cell signaling and membrane integrity.

Pathways

The involvement of VAPA is significant in lipid metabolism and membrane trafficking pathways. It interacts with proteins like VAMP (vesicle-associated membrane proteins) and SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes which are important for exocytosis and endocytosis processes. VAPA's interaction in these pathways supports the movement of materials across cellular membranes and its regulation of lipid exchange impacts the signaling pathways influencing cell growth and differentiation.

Abnormal VAPA function relates to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and some forms of motor neuron disease. In such conditions VAPA’s interaction with proteins like TDP-43 (TAR DNA-binding protein 43) becomes disrupted leading to protein aggregation and neuronal toxicity. Additionally disruptions in VAPA-associated lipid transport are linked to metabolic disorders like type 2 diabetes highlighting its critical role in proper cellular communication and metabolic balance.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44 (PubMed : 32344433, PubMed : 33124732). STARD3-VAPA interaction enables cholesterol transfer from the ER to endosomes (PubMed : 33124732). Via interaction with WDR44 participates in neosynthesized protein export (PubMed : 32344433). In addition, recruited to the plasma membrane through OSBPL3 binding (PubMed : 25447204). The OSBPL3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed : 25447204). With OSBPL3, may regulate ER morphology (PubMed : 16143324). May play a role in vesicle trafficking (PubMed : 11511104, PubMed : 19289470).

See full target information VAPA

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 15:5451-5461 PubMed39247600

2024

circTP63 promotes prostate cancer progression via miR-421/VAMP associated protein A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfeng Xu,Siwei Xu,Weihui Liu,Jiabi Chen,Longbo Cai,Wei Zhuang

Nature cell biology 26:378-392 PubMed38429475

2024

Combinatorial selective ER-phagy remodels the ER during neurogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa J Hoyer,Cristina Capitanio,Ian R Smith,Julia C Paoli,Anna Bieber,Yizhi Jiang,Joao A Paulo,Miguel A Gonzalez-Lozano,Wolfgang Baumeister,Florian Wilfling,Brenda A Schulman,J Wade Harper

The Journal of cell biology 222: PubMed37737955

2023

Peroxisomal ROS control cytosolic Mycobacterium tuberculosis replication in human macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Enrica Pellegrino,Beren Aylan,Claudio Bussi,Antony Fearns,Elliott M Bernard,Natalia Athanasiadi,Pierre Santucci,Laure Botella,Maximiliano G Gutierrez

Proceedings of the National Academy of Sciences of the United States of America 120:e2219790120 PubMed37307450

2023

Dynamic interactome of the MHC I peptide loading complex in human dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Barends,Nicole Koller,Christian Schölz,Verónica Durán,Berislav Bošnjak,Jennifer Becker,Marius Döring,Hanna Blees,Reinhold Förster,Ulrich Kalinke,Robert Tampé

Science advances 6: PubMed33328230

2020

The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Lu,Francesca W van Tartwijk,Julie Qiaojin Lin,Wilco Nijenhuis,Pierre Parutto,Marcus Fantham,Charles N Christensen,Edward Avezov,Christine E Holt,Alan Tunnacliffe,David Holcman,Lukas Kapitein,Gabriele S Kaminski Schierle,Clemens F Kaminski

EMBO reports 20:e46451 PubMed32048434

2019

Signal peptide peptidase-like 2c impairs vesicular transport and cleaves SNARE proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Alkmini A Papadopoulou,Stephan A Müller,Torben Mentrup,Merav D Shmueli,Johannes Niemeyer,Martina Haug-Kröper,Julia von Blume,Artur Mayerhofer,Regina Feederle,Bernd Schröder,Stefan F Lichtenthaler,Regina Fluhrer

EMBO reports 20: PubMed30733281

2019

Signal Peptide Peptidase-Like 2c (SPPL2c) impairs vesicular transport and cleavage of SNARE proteins.

Applications

Unspecified application

Species

Unspecified reactive species

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VAPA 抗体 [EPR13589(B)] - N-terminal

Anti-VAPA antibody [EPR13589(B)] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (AB181067)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (AB181067)

Western blot - Anti-VAPA antibody [EPR13589(B)] - N-terminal (AB181067)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (7)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise