Anti-VAP1 抗体 (ab42885)
Key features and details
- Rabbit polyclonal to VAP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-VAP1 antibody
VAP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VAP1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Rat VAP1 aa 150-250.
(Peptide available asab42884) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following Tissue Lysates: Lung (Rat), Lung (Mouse) and Lung (Human). This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab42885の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 85 kDa).
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特記事項 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 85 kDa). |
ターゲット情報
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機能
Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. -
組織特異性
Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis. -
配列類似性
Belongs to the copper/topaquinone oxidase family. -
翻訳後修飾
Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
N- and O-glycosylated. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8639 Human
- Entrez Gene: 11754 Mouse
- Entrez Gene: 29473 Rat
- Omim: 603735 Human
- SwissProt: Q16853 Human
- SwissProt: O70423 Mouse
- SwissProt: O08590 Rat
- Unigene: 198241 Human
see all -
別名
- Amine oxidase, copper containing 3 (vascular adhesion protein 1) antibody
- AOC3 antibody
- AOC3_HUMAN antibody
see all
画像
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IHC image of VAP1 antibody staining in a section of formalin-fixed paraffin-embedded normal human lung* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab42885, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : Anti-VAP1 antibody (ab42885) at 1 µg/ml
Lane 1 : Lung (Rat) Tissue Lysate
Lane 2 : Lung (Mouse) Whole Cell Lysate - normal tissue
Lane 3 : Lung (Human) Tissue Lysate - Fetal Normal Tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.
VAP1 has a predicted molecular weight of 85 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). The observed 100kDa band is consistent with other commercially avaliable antibodies.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab42885 は 6 報の論文で使用されています。
- Yao D et al. Accumulation of formaldehyde causes motor deficits in an in vivo model of hindlimb unloading. Commun Biol 4:933 (2021). PubMed: 34413463
- Becchi S et al. Inhibition of vascular adhesion protein 1 protects dopamine neurons from the effects of acute inflammation and restores habit learning in the striatum. J Neuroinflammation 18:233 (2021). PubMed: 34654450
- Romauch M Zinc-a2-glycoprotein as an inhibitor of amine oxidase copper-containing 3. Open Biol 10:190035 (2020). PubMed: 32315567
- Yoshida S et al. Proteolytic cleavage of vascular adhesion protein-1 induced by vascular endothelial growth factor in retinal capillary endothelial cells. Jpn J Ophthalmol 62:256-264 (2018). PubMed: 29392528
- Cha YJ et al. Site-specific expression of amine oxidases in breast cancer metastases. Tumour Biol 40:1010428318776822 (2018). PubMed: 29775160
- Murata M et al. Soluble Vascular Adhesion Protein-1 Mediates Spermine Oxidation as Semicarbazide-Sensitive Amine Oxidase: Possible Role in Proliferative Diabetic Retinopathy. Curr Eye Res 42:1674-1683 (2017). PubMed: 28937866