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Anti-VAP1 抗体 [7-88] (ab81673)

Submit a review Q&A (2)References (7)
Immunohistochemistry (Frozen sections) - Anti-VAP1 antibody [7-88] (ab81673)

    Key features and details

    • Rat monoclonal [7-88] to VAP1
    • Suitable for: IHC-Fr
    • Reacts with: Mouse
    • Isotype: IgG2b

    製品の概要

    • 製品名

      Anti-VAP1 antibody [7-88]
      VAP1 一次抗体 製品一覧

    • 製品の詳細

      Rat monoclonal [7-88] to VAP1

    • 由来種

      Rat

    • アプリケーション

      適用あり: IHC-Frmore details

    • 種交差性

      交差種: Mouse

    • 免疫原

      Tissue, cells or virus corresponding to Mouse VAP1.

    • ポジティブ･コントロール

      • Mouse spleen Mouse VAP-1-transfected CHO cells

    • 特記事項


      Inhibits migration of granulocytes and monocytes in acute models of inflammation.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab81673の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-Fr
    1/50.
    特記事項
    IHC-Fr
    1/50.

    ターゲット情報

    • 機能

      Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.

    • 組織特異性

      Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis.

    • 配列類似性

      Belongs to the copper/topaquinone oxidase family.

    • 翻訳後修飾

      Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
      N- and O-glycosylated.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Amine oxidase, copper containing 3 (vascular adhesion protein 1) antibody
        • AOC3 antibody
        • AOC3_HUMAN antibody
        • Copper amine oxidase antibody
        • HPAO antibody
        • Membrane copper amine oxidase antibody
        • Membrane primary amine oxidase antibody
        • Semicarbazide sensitive amine oxidase antibody
        • Semicarbazide-sensitive amine oxidase antibody
        • SSAO antibody
        • VAP-1 antibody
        • Vascular adhesion protein 1 antibody
        • VP97 antibody
        see all

      画像

      • Immunohistochemistry (Frozen sections) - Anti-VAP1 antibody [7-88] (ab81673)
        Immunohistochemistry (Frozen sections) - Anti-VAP1 antibody [7-88] (ab81673)
        ab81673 staining VAP1 in mouse muscle tissue by Immunohistochemistry (Frozen sections).

      参考文献 (7)

      ab81673 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab81673 は 7 報の論文で使用されています。

      • Dong H  et al. Identification of a regulatory pathway inhibiting adipogenesis via RSPO2. Nat Metab 4:90-105 (2022). PubMed: 35027768
      • Hu Q  et al. MicroRNA-137 exerts protective effects on hypoxia-induced cell injury by inhibiting autophagy/mitophagy and maintaining mitochondrial function in breast cancer stem-like cells. Oncol Rep 44:1627-1637 (2020). PubMed: 32945512
      • Schwalie PC  et al. A stromal cell population that inhibits adipogenesis in mammalian fat depots. Nature 559:103-108 (2018). PubMed: 29925944
      • Yoshikawa N  et al. Blockade of vascular adhesion protein-1 attenuates choroidal neovascularization. Mol Vis 18:593-600 (2012). ICC/IF ; Mouse . PubMed: 22419852
      • Merinen M  et al. Vascular adhesion protein-1 is involved in both acute and chronic inflammation in the mouse. Am J Pathol 166:793-800 (2005). Flow Cyt, Functional Studies, IHC-Fr ; Mouse . PubMed: 15743791
      • Bonder CS  et al. Rules of recruitment for Th1 and Th2 lymphocytes in inflamed liver: a role for alpha-4 integrin and vascular adhesion protein-1. Immunity 23:153-63 (2005). IHC-Fr ; Mouse . PubMed: 16111634
      • Abella A  et al. Adipocytes release a soluble form of VAP-1/SSAO by a metalloprotease-dependent process and in a regulated manner. Diabetologia 47:429-38 (2004). IP ; Mouse . PubMed: 14968297

      レビューと Q&A

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      レビューを送る 質問を送る

      1-2 of 2 Abreviews or Q&A

      Question

      please find here below our customer's answer:
      Tumor tissues are about 1cm in diameter, therefore the entire tissue had to be fixed for at least 2 hs, then it was incubated in sucrose 10%-20%-30% in order to preserve the tissue from the freezing. Incubation time depends on the tissue: when the sucrose solution spreads into the entire tissue, the formalin is washed away and the tissue moves from the top to the bottom of the solution: after this, the solution can be changed. Tissue was briefly fixed again (10 minutes, in the same solution of 10% formalin buffered), before the application of labelling protocol. This is a standard protocol to fix a tissue; it is possible that the tissue results over-fixed, but we never had problems to highlight a marker with an antibody. It is possible that this particular antigen requires an antigen retrieval procedure in addition to the standard labelling protocol. Could you suggest me some antigen retrieval protocol?

      Kind regards

      Read More
      Answer

      Thank you for having followed this up with the customer.

      Indeed, the protocol seems to me to be intermediate in the fixation with paraffin embedded tissues, where the tissues are often fixed overnight in PFA, and with frozen sections, where the sections are just postfixed.
      We do have unfortunately no experience with this antibody on paraffin embedded orotherlong term PFA fixed tissue(such aswholemount)and I can not recommend on particular antigen retrieval method. I have not found a reference in this regards in the literature neither.
      I can not guarantee that this will work, however there is neverthelessa good chance that antigen retrieval couldallow antibody staining.I would therefore recommend the customer to test different antigen retrievals, such as heat induced antigen retrieval with low and high pH (e.g. with Citrate and EDTA buffers). Here is publication comparing different antigen retrievals- Emoto et al., J Histochem Cytochem. 2005 Nov;53(11):1311-21

      I hope this makes sense. Please do let me know how the customer is proceeding. Ifthe antigen retrieval will not allow good results, I will arrange for a replacement or credit.

      Please let me knowif you have any questions or concerns.

      I wish you a great weekend!

      Read More

      Question


      1)Antibody storage conditions (temperature/reconstitution etc)
      +4 C not aliquoted

      2)Description of the problem (high background, low signal, non-specific satining etc.)
      No signal
      3)Sample (Species/Tissue/Cell Type/Cell Line etc.)
      Mouse liver sections (frozen sections in isopentane cooled in liquid nitrogen; tissue pre-fixed in buffered formalin 10% and then incubated in sucrose 10-20-30%)

      4)Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)
      Buffered formalin 10% 20 min at RT+ incubation in PBS+Glycine 0.1M 20 min
      Tried also Acetone:ethanol 3:1 10 min at RT + incubation in PBS 10min
      5)Antigen retrieval (Enzymatic method, Heat mediated technique etc.)
      NO

      6)Permeabilization step
      Yes, with incubation in PBS+Triton X-100 0.05% 20min

      7)Blocking conditions (Buffer/time period, Blocking agent etc.)
      Yes, with incubation in PBS+BSA 3% 30min

      8)Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
      Rat monoclonal anti-VAP1 diluted 1:50 in PBS, incubated for 3,5hs at RT in wet chamber

      9)Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
      Goat anti-rat FITC-conjugated (cod.AP183F, Chemicon), diluted 1:200 in PBS, incubated for 1h at RT

      10)Detection method
      Immunofluorescence microscopy

      11)Positive and negative controls used (please specify)
      Negative control without primary antibody

      12) How many times have you tried the IHC?
      4 times (immunofluorescence)

      13)Have you run a "No Primary" control?
      Yes

      14)Do you obtain the same results every time?
      Yes

      15)What steps have you altered?
      I tried to avoid permeabilisation and blocking steps, to incubate with primary antibody overnight, to do different fixations (formalin and acetone:ethanol).
      Additional Notes

      Read More
      Answer

      Thank you very much for contacting us and liaising with this customer.

      I can confirm that we will accept this complaint under our Abpromise guarantee and thank you for looking after it.

      I have looked through the protocol and have one question:

      It states that the sections have been prefixed in formalin. Can you please provide more information on this step? Could it be that the samples have been overfixed and an antigen retrieval would be necessary?

      I am looking forward to your reply.

      Read More

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