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リコンビナントRabMAb

Anti-VAMP1 抗体 [EPR7325(2)] (ab151712)

Reviews (1) Submit a question References (4)
Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7325(2)] to VAMP1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-VAMP1 antibody [EPR7325(2)]
    VAMP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR7325(2)] to VAMP1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse brain, Rat brain, Human cerebellum lysate. IHC-P: Human brain

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab151712の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF or IP.

ターゲット情報

  • 機能

    Involved in the targeting and/or fusion of transport vesicles to their target membrane.

  • 組織特異性

    Nervous system, skeletal muscle and adipose tissue.

  • 配列類似性

    Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.

  • 細胞内局在

    Mitochondrion outer membrane; Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome and Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • DKFZp686H12131 antibody
    • SYB 1 antibody
    • SYB1 antibody
    • Synaptobrevin 1 antibody
    • Synaptobrevin-1 antibody
    • Synaptobrevin1 antibody
    • VAMP 1 antibody
    • VAMP-1 antibody
    • Vamp1 antibody
    • VAMP1_HUMAN antibody
    • Vesicle associated membrane protein 1 antibody
    • Vesicle associated membrane protein 1 synaptobrevin 1 antibody
    • Vesicle-associated membrane protein 1 antibody
    see all

画像

  • Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    All lanes : Anti-VAMP1 antibody [EPR7325(2)] (ab151712) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa
    Observed band size: 16 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds

  • Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Anti-VAMP1 antibody [EPR7325(2)] (ab151712) at 1/1000 dilution + Human cerebellum lysate at 10 µg

    Predicted band size: 13 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab151712 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab151712 showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab151712 showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab151712 showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151712 showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

参考文献 (4)

ab151712 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab151712 は 4 報の論文で使用されています。

  • Wu Z  et al. Dexmedetomidine Mitigates Microglial Activation Associated with Postoperative Cognitive Dysfunction by Modulating the MicroRNA-103a-3p/VAMP1 Axis. Neural Plast 2022:1353778 (2022). PubMed: 35494481
  • Du H  et al. Ablation of GSDMD Attenuates Neurological Deficits and Neuropathological Alterations After Traumatic Brain Injury. Front Cell Neurosci 16:915969 (2022). PubMed: 35669106
  • Ding J  et al. Trilobatin Alleviates Cognitive Deficits and Pathologies in an Alzheimer's Disease Mouse Model. Oxid Med Cell Longev 2021:3298400 (2021). PubMed: 34777683
  • Her LS  et al. miR-196a Enhances Neuronal Morphology through Suppressing RANBP10 to Provide Neuroprotection in Huntington's Disease. Theranostics 7:2452-2462 (2017). WB ; Mouse . PubMed: 28744327

レビューと Q&A

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Western blot abreview for Anti-VAMP1 antibody [EPR7325(2)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (primary culture hippocampus cell)
Gel Running Conditions
Non-reduced Denaturing (15% SDS-PAGE)
Loading amount
15 µg
Treatment
RIPA & 1%Protease inhibitor for 15min on ice
Specification
primary culture hippocampus cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Oct 10 2018

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