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Anti-Vaccinia Virus 抗体 (ab35219)

Reviews (4)Q&A (2)References (33)

Key features and details

  • Rabbit polyclonal to Vaccinia Virus
  • Suitable for: ELISA, IHC-Fr, WB, IHC-P, ICC/IF
  • Reacts with: Vaccinia virus
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Vaccinia Virus antibody
    Vaccinia Virus 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Vaccinia Virus

  • 由来種

    Rabbit

  • 特異性

    This antibody reacts with purified Virions. It does not react with uninfected cells.

  • アプリケーション

    適用あり: ELISA, IHC-Fr, WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Vaccinia virus

  • 免疫原

    Tissue, cells or virus corresponding to Vaccinia Virus. Vaccinia virus, New York City Board of Health (NYCBOH) strain.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    This antibody is greater than 95% pure.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab35219の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
1/500 - 1/2000.
IHC-Fr
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 25093734
IHC-P (2)
Use at an assay dependent concentration. PubMed: 25093734
ICC/IF (1)
Use at an assay dependent concentration. PubMed: 22615950
特記事項
ELISA
1/500 - 1/2000.
IHC-Fr
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 25093734
IHC-P
Use at an assay dependent concentration. PubMed: 25093734
ICC/IF
Use at an assay dependent concentration. PubMed: 22615950

ターゲット情報

  • 関連性

    Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.

  • 別名

    • Orthopoxvirus antibody

参考文献 (33)

ab35219 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab35219 は 33 報の論文で使用されています。

  • Ottolino-Perry K  et al. Vaccinia virus and peptide-receptor radiotherapy synergize to improve treatment of peritoneal carcinomatosis. Mol Ther Oncolytics 29:44-58 (2023). PubMed: 37180034
  • Rodríguez-Cuadrado FJ  et al. Clinical, histopathologic, immunohistochemical, and electron microscopic findings in cutaneous monkeypox: A multicenter retrospective case series in Spain. J Am Acad Dermatol 88:856-863 (2023). PubMed: 36581043
  • Lee N  et al. Generation of novel oncolytic vaccinia virus with improved intravenous efficacy through protection against complement-mediated lysis and evasion of neutralization by vaccinia virus-specific antibodies. J Immunother Cancer 11:N/A (2023). PubMed: 36717184
  • Koch J  et al. Establishing a New Platform to Investigate the Efficacy of Oncolytic Virotherapy in a Human Ex Vivo Peritoneal Carcinomatosis Model. Viruses 15:N/A (2023). PubMed: 36851574
  • Yang A  et al. Peritoneal-directed chimeric oncolytic virus CF17 prevents malignant ascites and improves survival in gastric cancer peritoneal metastases. Mol Ther Oncolytics 31:100734 (2023). PubMed: 37915757
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-Vaccinia Virus antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Orthopoxvirus Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
75000 cells
Treatment
Vaccinia Virus Western Reserve, 16-18h
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 05 2023

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vaccinia Virus antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Monkeypox infected - HFFs)
Permeabilization
Yes - 0.1% triton
Specification
Monkeypox infected - HFFs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Or Alfi

Verified customer

投稿 Oct 04 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vaccinia Virus antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
Yes - H2O2
Specification
Skin
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 25 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vaccinia Virus antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cross section of mouse eye, looked at cornea)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS EDTA pH 9
Permeabilization
No
Specification
Cross section of mouse eye, looked at cornea
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Inna Larsen

Verified customer

投稿 Dec 29 2015

Question

I am planning to order a Vaccinia antibody ab117453 and have few questions to address.

In the product datasheet I saw that the antibody recognizes A27L fusion protein of Vaccinia virus Lister strain. However, I work with Vaccinia virus Western Reserve strain and my question is whether I can use that same antibody to detect my virus strain.

Second, how specific is the antibody in terms of binding to other cellular components and components of ECM since I am planning to use it for staining tumor samples.

Read More
Answer


Unfortunately, none of the anti-Vaccinia Virus antibodies we have has been tested for cross-reactivity with Western Reserve Strain.


ab117453 has been tested and cross-reacts with 3 other Vaccinia strains and also with distantly related Monkeypox.

ab28580 does not react with human cellular components.

ab35219 is known to be reactive to the Lister and MVA strains of Vaccinia. It only binds to the virus components and does not react to uninfected cells.

ab156854 has only been tested in the Lister and NYCBH strains. This antibody does not react with human cellular components.

Read More

Question



Inquiry: Hi, I am looking for few different antibodies against vaccinia virus. Firstly I am looking for monoclonal antibodies against A27L, H3L and and D8L. I am also looking for antibodies that will neutralize the purified form of vaccinia virus, and stop it from infecting cells in vitro. Please get back to me as soon as possible if you have any products to offer me.

Read More
Answer

Thank you for contacting us.

We only have two monoclonal antibodies against the vaccinia virus in our catalogue.

I would recommend using ab21098 - Mouse monoclonal [TV46] to Vaccinia Virus (https://www.abcam.com/ab21098) to recognise A27L.

I have not found an antibody against H3L and D8L in our catalogue. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are:
www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

As for the antibody that will neutralize the purified forms of the vaccinia virus we do not have any antibodies tested as neutralising antibodies. However, if you would like to buy and test either one of our mouse monoclonal antibodies (ab48569 or ab21098) as a neutralising antibody, we can offer you a discount off your next order.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

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