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AB84531

Anti-USP47 抗体

Anti-USP47 antibody

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(1 Publication)

Rabbit Polyclonal USP47 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human USP47.

別名を表示する

Ubiquitin carboxyl-terminal hydrolase 47, Deubiquitinating enzyme 47, Ubiquitin thioesterase 47, Ubiquitin-specific-processing protease 47, USP47

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)

ab84531 at a 1 : 500 dilution, staining human USP47 in basal cell carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection : DAB staining using an Immunohistochemistry Accessory Kit.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)

ab84531 at a 1 : 500 dilution, staining human USP47 in prostate carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection : DAB staining using an Immunohistochemistry Accessory Kit

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)

ab84531 at a 1 : 100 dilution, staining human USP47 in a FFPE section of prostate adenocarcinoma, using Immunofluorescence. Detection : Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1 : 500.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)

ab84531 at a 1 : 100 dilution, staining mouse USP47 in teratoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection : DAB staining using an Immunohistochemistry Accessory Kit.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

IHC-P

applications

免疫原

Synthetic Peptide within Human USP47. The exact immunogen used to generate this antibody is proprietary information.

Q96K76

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Affinity purified using an epitope specific to USP47 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
バッファー組成
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

USP47 also known as Ubiquitin Specific Peptidase 47 plays a role in deubiquitinating proteins by removing ubiquitin molecules from them. It is a member of the ubiquitin-specific proteases with a molecular mass of approximately 159 kDa. USP47 expresses widely in various tissues including the brain lungs and liver.
Biological function summary

The function of USP47 focuses on maintaining levels of key proteins that require deubiquitination. It stabilizes beta-Catenin and other regulatory proteins by protecting them from proteasomal degradation. As a part of the larger ubiquitin-proteasome system USP47 works with other deubiquitinating enzymes but forms no stable complex with them. Its action ensures the regulation of protein homeostasis impacting cell cycle progression and DNA damage response.

Pathways

USP47 interacts with the Wnt signaling pathway and the DNA damage repair pathway. By controlling the stability of beta-Catenin USP47 influences the Wnt signaling which plays a role in cell growth and differentiation. In the context of DNA repair USP47 interacts with other proteins like Flap endonuclease 1 (FEN1) to contribute to genome integrity by processing DNA lesions and maintaining genomic stability.

USP47 shows involvement with cancer and neurological disorders. Abnormal USP47 activity can lead to uncontrolled cell proliferation due to impacted beta-Catenin levels linking it to cancerous transformations. In neurological disorders USP47's effect on protein homeostasis may connect with proteins like Tau suggesting a role in neurodegenerative disorders where protein aggregation occurs.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.
See full target information USP47
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1026 PubMed39863586

2025

CHD6 has poly(ADP-ribose)- and DNA-binding domains and regulates PARP1/2-trapping inhibitor sensitivity via abasic site repair.

Applications

Unspecified application

Species

Unspecified reactive species

Luc Provencher,Wilson Nartey,Peter M Brownlee,Austin W Atkins,Jean-Philippe Gagné,Lou Baudrier,Nicholas S Y Ting,Cortt G Piett,Shujuan Fang,Dustin D Pearson,Shaun Moore,Pierre Billon,Zachary D Nagel,Guy G Poirier,Gareth J Williams,Aaron A Goodarzi
View all publications
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代替製品

Primary Antibodies

AB72143

Anti-USP47 antibody

Immunoprecipitation - Anti-USP47 antibody (AB72143)

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組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Proteins & Peptides

AB162936

Recombinant Human USP47 protein

SDS-PAGE - Recombinant Human USP47 protein (AB162936)

  • 0
  • 0
Primary Antibodies

AB236632

Anti-HA tag antibody [EPR22819-101]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (AB236632)

  • 3
  • 1
Primary Antibodies

AB89901

Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (AB89901)

  • 5
  • 43

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