Anti-USP4 抗体 (ab245654)
Key features and details
- Rabbit polyclonal to USP4
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-USP4 antibody
USP4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to USP4 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Monkey, Orangutan, Giant Panda
-
免疫原
Synthetic peptide within Human USP4 aa 925-963. The exact sequence is proprietary. AAB72237.1
Database link: Q13107 -
ポジティブ･コントロール
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245654 was affinity purified using an epitope specific to USP4 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245654の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 109 kDa.
|
| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 109 kDa. |
ターゲット情報
-
機能
Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER. -
組織特異性
Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung (at protein level). Expressed in the hippocampal neurons. -
配列類似性
Belongs to the peptidase C19 family. USP4 subfamily.
Contains 1 DUSP domain. -
翻訳後修飾
Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated. -
細胞内局在
Cytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type. - Information by UniProt
-
参照データベース
- Entrez Gene: 7375 Human
- Entrez Gene: 22258 Mouse
- Omim: 603486 Human
- SwissProt: Q08AK7 Human
- SwissProt: Q08AK8 Human
- SwissProt: Q13107 Human
- SwissProt: P35123 Mouse
- SwissProt: Q3TEX8 Mouse
see all -
別名
- Deubiquitinating enzyme 4 antibody
- MGC149848 antibody
- MGC149849 antibody
see all
画像
-
Western blot - Anti-USP4 antibody (ab245654)All lanes : Anti-USP4 antibody (ab245654) at 0.4 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 109 kDa
Exposure time: 30 secondsPrepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-USP4 antibody (ab245654)USP4 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245654 used for IP at 6 µg per reaction. For WB 1 µg/ml.
Lane 1: ab245654 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Chemiluminescence detection: 30 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab245654 は論文での使用が確認できていません。