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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-USP28 抗体 [EPR4250(2)] (ab110744)

Reviews (1) Submit a question References (2)
Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
  • Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
  • Anti-USP28 antibody [EPR4250(2)] (ab110744)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4250(2)] to USP28
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-USP28 antibody [EPR4250(2)]
    USP28 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4250(2)] to USP28

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • 293T, SW480, HeLa, and A431 cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab110744の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 122 kDa.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 122 kDa.
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.

  • 配列類似性

    Belongs to the peptidase C19 family. USP28 subfamily.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.

  • 翻訳後修飾

    Degradaded upon nickel ion level or hypoxia exposure.
    Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR.

  • 細胞内局在

    Nucleus > nucleoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Deubiquitinating enzyme 28 antibody
    • KIAA1515 antibody
    • Ubiquitin carboxyl terminal hydrolase 28 antibody
    • Ubiquitin carboxyl-terminal hydrolase 28 antibody
    • ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody
    • Ubiquitin specific peptidase 28 antibody
    • Ubiquitin specific processing protease 28 antibody
    • Ubiquitin specific protease 28 antibody
    • Ubiquitin thioesterase 28 antibody
    • Ubiquitin thiolesterase 28 antibody
    • Ubiquitin-specific-processing protease 28 antibody
    • UBP28_HUMAN antibody
    • USP 28 antibody
    • USP28 antibody
    • USP28 protein antibody
    see all

画像

  • Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
    Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: USP28 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab110744 observed at 128 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab110744 was shown to specifically react with USP28 when USP28 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP28 knockout samples were subjected to SDS-PAGE.  Ab110744 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
    Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
    All lanes : Anti-USP28 antibody [EPR4250(2)] (ab110744) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 122 kDa

  • Anti-USP28 antibody [EPR4250(2)] (ab110744)
    Anti-USP28 antibody [EPR4250(2)] (ab110744)

参考文献 (2)

ab110744 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab110744 は 2 報の論文で使用されています。

  • Tang M  et al. Antileukemic effect of caffeic acid 3,4-dihydroxyphenetyl ester. Evidences for its mechanisms of action. Phytomedicine 80:153383 (2021). PubMed: 33091855
  • Wrigley JD  et al. Identification and Characterization of Dual Inhibitors of the USP25/28 Deubiquitinating Enzyme Subfamily. ACS Chem Biol 12:3113-3125 (2017). PubMed: 29131570

レビューと Q&A

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Western blot abreview for Anti-USP28 antibody [EPR4250(2)]

 Poor
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Xenopus laevis Cell lysate - whole cell (egg extracts)
Specification
egg extracts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Sep 02 2014

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