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リコンビナントRabMAb

Anti-USP25 抗体 [EPR15019] (ab187156)

Submit a review Submit a question References (22)
Western blot - Anti-USP25 antibody [EPR15019] (ab187156)
  • Western blot - Anti-USP25 antibody [EPR15019] (ab187156)
  • Flow Cytometry (Intracellular) - Anti-USP25 antibody [EPR15019] (ab187156)
  • Anti-USP25 antibody [EPR15019] (ab187156)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15019] to USP25
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-USP25 antibody [EPR15019]
    USP25 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15019] to USP25

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • 293, A673, Raw264.7, PC-12 and BxPC-3 cell lysate. A673 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187156の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
特記事項
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).

ターゲット情報

  • 機能

    Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
    The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.

  • 組織特異性

    Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific.

  • 配列類似性

    Belongs to the peptidase C19 family.
    Contains 1 UBA-like domain.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.

  • 翻訳後修飾

    Acetylated.
    Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially by SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
    Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
    Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.

  • 細胞内局在

    Cytoplasm and Cytoplasm. Nucleus. Some transient punctuate nuclear location in myotubes during myocyte development.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Deubiquitinating enzyme 25 antibody
      • Ubiquitin carboxyl-terminal hydrolase 25 antibody
      • Ubiquitin specific peptidase 25 antibody
      • ubiquitin specific protease 25 antibody
      • ubiquitin specific protease USP25 antibody
      • Ubiquitin thiolesterase 25 antibody
      • Ubiquitin-specific-processing protease 25 antibody
      • UBP25_HUMAN antibody
      • USP on chromosome 21 antibody
      • USP21 antibody
      • USP25 antibody
      see all

    画像

    • Western blot - Anti-USP25 antibody [EPR15019] (ab187156)
      Western blot - Anti-USP25 antibody [EPR15019] (ab187156)
      All lanes : Anti-USP25 antibody [EPR15019] (ab187156) at 1/10000 dilution

      Lane 1 : 293 cell lysate
      Lane 2 : A673 cell lysate
      Lane 3 : BxPC-3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 122 kDa
      Observed band size: 122 kDa



      Blocking/ Dilution buffer: 5% NFDM /TBST.

    • Western blot - Anti-USP25 antibody [EPR15019] (ab187156)
      Western blot - Anti-USP25 antibody [EPR15019] (ab187156)
      All lanes : Anti-USP25 antibody [EPR15019] (ab187156) at 1/1000 dilution

      Lane 1 : Raw264.7 cell lysate
      Lane 2 : PC-12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 122 kDa



      Blocking/ Dilution buffer: 5% NFDM /TBST.

    • Flow Cytometry (Intracellular) - Anti-USP25 antibody [EPR15019] (ab187156)
      Flow Cytometry (Intracellular) - Anti-USP25 antibody [EPR15019] (ab187156)

      Intracellular Flow Cytometry analysis of USP25 expression in 2% paraformaldehyde-fixed A673 cells using ab187156 at 1/60 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. 

       

       

    • Anti-USP25 antibody [EPR15019] (ab187156)
      Anti-USP25 antibody [EPR15019] (ab187156)

    参考文献 (22)

    ab187156 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab187156 は 22 報の論文で使用されています。

    • Chan WC  et al. Accelerating inhibitor discovery for deubiquitinating enzymes. Nat Commun 14:686 (2023). WB ; Human . PubMed: 36754960
    • Zhao Y  et al. USP25 inhibits renal fibrosis by regulating TGFβ-SMAD signaling pathway in Ang II-induced hypertensive mice. Biochim Biophys Acta Mol Basis Dis 1869:166713 (2023). PubMed: 37059312
    • Vozandychova V  et al. Modified activities of macrophages' deubiquitinating enzymes after Francisella infection. Front Immunol 14:1252827 (2023). PubMed: 37841261
    • Liu X  et al. USP25 Deficiency Exacerbates Acute Pancreatitis via Up-Regulating TBK1-NF-κB Signaling in Macrophages. Cell Mol Gastroenterol Hepatol 14:1103-1122 (2022). PubMed: 35934222
    • Akopian D  et al. Co-adaptor driven assembly of a CUL3 E3 ligase complex. Mol Cell 82:585-597.e11 (2022). PubMed: 35120648
    View all Publications for this product

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