Anti-USP19 抗体 (ab68527)
Key features and details
- Mouse polyclonal to USP19
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-USP19 antibody
USP19 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to USP19 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Full-length Human protein (NP_006668.1, aa 1-1318).
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ポジティブ･コントロール
- USP19-transfected 293T cells lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68527の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 175 kDa (predicted molecular weight: 145 kDa).
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 175 kDa (predicted molecular weight: 145 kDa). |
ターゲット情報
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機能
Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates. -
配列類似性
Belongs to the peptidase C19 family.
Contains 2 CS domains.
Contains 1 MYND-type zinc finger. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10869 Human
- Omim: 614471 Human
- SwissProt: O94966 Human
- Unigene: 255596 Human
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別名
- 8430421I07Rik antibody
- AI047774 antibody
- Deubiquitinating enzyme 19 antibody
see all
画像
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Western blot - Anti-USP19 antibody (ab68527)All lanes : Anti-USP19 antibody (ab68527) at 1/500 dilution
Lane 1 : transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 145 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (2)
ab68527 は 2 報の論文で使用されています。
- Zhang A et al. USP33 deubiquitinates and stabilizes HIF-2alpha to promote hypoxia response in glioma stem cells. EMBO J 41:e109187 (2022). PubMed: 35191554
- Bernadotte A et al. In silico identification and biochemical characterization of the human dicarboxylate clamp TPR protein interaction network. FEBS Open Bio 8:1830-1843 (2018). PubMed: 30410862
