Anti-USP15 抗体 (ab4850)
Key features and details
- Goat polyclonal to USP15
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-USP15 antibody
USP15 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to USP15 -
由来種
Goat -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Cow, Dog, Pig -
免疫原
Synthetic peptide corresponding to Human USP15 aa 970-981 (C terminal).
Sequence:NDIENENCMHTN
Database link: Q9Y4E8 -
特記事項
Gene Ontology terms - ubiquitin-specific protease activity; cysteine-type endopeptidase activity; deubiquitination.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4850の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 2.5 - 3.8 µg/ml.
Perform heat mediated antigen retrieval with citrate buffer (pH6). |
特記事項 |
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IHC-P
Use a concentration of 2.5 - 3.8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer (pH6). |
ターゲット情報
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機能
Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway. -
組織特異性
Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues. -
配列類似性
Belongs to the peptidase C19 family.
Contains 1 DUSP domain. -
翻訳後修飾
Phosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome.
Ubiquitinated, leading to degradation by the proteasome. - Information by UniProt
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参照データベース
- Entrez Gene: 538284 Cow
- Entrez Gene: 9958 Human
- Entrez Gene: 171329 Rat
- Omim: 604731 Human
- SwissProt: Q9Y4E8 Human
- Unigene: 434951 Human
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別名
- Deubiquitinating enzyme 15 antibody
- Deubiquitinating enzyme antibody
- EC 3 1 2 15 antibody
see all
画像
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Immunohistochemical analysis of paraffin embedded Human Liver labeling USP15 with ab4850 at 2.5-3.8µg/ml. The image shows patterned intracellular staining in hepatocytes.
Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab4850 は 1 報の論文で使用されています。
- Zhao Y et al. USP15 Enhances Re-epithelialization Through Deubiquitinating EIF4A1 During Cutaneous Wound Repair. Front Cell Dev Biol 8:529 (2020). PubMed: 32671073