Anti-USP14/TGT 抗体 (ab71165)
Key features and details
- Rabbit polyclonal to USP14/TGT
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-USP14/TGT antibody
USP14/TGT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to USP14/TGT -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey
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免疫原
Synthetic peptide corresponding to Human USP14/TGT aa 350-400 (internal sequence).
Database link: NP_005142.1 -
ポジティブ･コントロール
- Whole cell lysates from Hela cells and 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab71165の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). -
配列類似性
Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
Contains 1 ubiquitin-like domain. -
細胞内局在
Cytoplasm. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 455325 Chimpanzee
- Entrez Gene: 9097 Human
- Omim: 607274 Human
- SwissProt: P60051 Chimpanzee
- SwissProt: P54578 Human
- Unigene: 464416 Human
- Unigene: 707058 Human
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別名
- Deubiquitinating enzyme 14 antibody
- TGT antibody
- tRNA guanine transglycosylase 60 kD subunit antibody
see all
画像
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Western blot - Anti-USP14/TGT antibody (ab71165)All lanes : Anti-USP14/TGT antibody (ab71165) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 28 kDa, 40 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP14/TGT antibody (ab71165)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP14/TGT with ab71165 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-USP14/TGT antibody (ab71165)Detection of Human USP14/TGT by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab71165 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab71165 は 4 報の論文で使用されています。
- Hong H et al. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma. Proteomics 23:e2200432 (2023). PubMed: 36625413
- Chen XY et al. The m6A Reader YTHDF1 Facilitates the Tumorigenesis and Metastasis of Gastric Cancer via USP14 Translation in an m6A-Dependent Manner. Front Cell Dev Biol 9:647702 (2021). PubMed: 33791305
- Lei J et al. The prognostic value of USP14 and PSMD14 expression in non-small cell lung cancer. Ann Transl Med 9:1019 (2021). PubMed: 34277819
- Chandrasekaran P et al. HIV-1 Nef down-modulates C-C and C-X-C chemokine receptors via ubiquitin and ubiquitin-independent mechanism. PLoS One 9:e86998 (2014). PubMed: 24489825