Anti-USP1 抗体 (ab84772)
Key features and details
- Rabbit polyclonal to USP1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-USP1 antibody
USP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to USP1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residue 450 and 500 of human USP1 (NP_003359.3)
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ポジティブ･コントロール
- Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Prostate Carcinoma, Small Cell Lung Cancer
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab84772 was affinity purified using an epitope specific to USP1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84772の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
| 特記事項 |
|---|
Epitope exposure is recommended.
Epitope exposure with citrate or tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. -
配列類似性
Belongs to the peptidase C19 family. -
発生段階
Cell cycle-regulated. Highest level during S phase. -
翻訳後修飾
Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.
Ubiquitinated; leading to its subsequent proteasomal degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 516328 Cow
- Entrez Gene: 7398 Human
- Entrez Gene: 230484 Mouse
- Entrez Gene: 313387 Rat
- Omim: 603478 Human
- SwissProt: Q29RP1 Cow
- SwissProt: O94782 Human
- SwissProt: Q8BJQ2 Mouse
see all -
別名
- Deubiquitinating enzyme 1 antibody
- hUBP antibody
- Ubiquitin carboxyl terminal hydrolase 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP1 antibody (ab84772)Staining of USP1 by Immunohistochemistry on a formalin fixed, paraffin embedded section of human thyroid carcinoma using a 1/250 dilution of ab84772. Detection: DAB staining
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab84772 は 1 報の論文で使用されています。
- Mussell A et al. USP1 Regulates TAZ Protein Stability Through Ubiquitin Modifications in Breast Cancer. Cancers (Basel) 12:N/A (2020). PubMed: 33114077