Anti-USO1 抗体 (ab184014)
Key features and details
- Rabbit polyclonal to USO1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-USO1 antibody
USO1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to USO1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment within Human USO1 aa 717-962. The exact sequence is proprietary.
Database link: O60763 -
ポジティブ･コントロール
- WB: Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, HEK-293T, A431 , HeLa , HepG2 whole cell lysates, IF: A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184014の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 108 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 108 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. -
配列類似性
Belongs to the VDP/USO1/EDE1 family.
Contains 10 ARM repeats. -
ドメイン
Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain. -
翻訳後修飾
Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation. -
細胞内局在
Cytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 8615 Human
- Entrez Gene: 56041 Mouse
- Omim: 603344 Human
- SwissProt: O60763 Human
- SwissProt: Q9Z1Z0 Mouse
- Unigene: 292689 Human
- Unigene: 15868 Mouse
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別名
- General vesicular transport factor antibody
- General vesicular transport factor p115 antibody
- P115 antibody
see all
画像
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Western blot - Anti-USO1 antibody (ab184014)All lanes : Anti-USO1 antibody (ab184014) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 108 kDa7.5% SDS PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-USO1 antibody (ab184014)Immunofluorescence analysis of methanol fixed A431 (human epidermoid carcinoma cell line) cells labelling USO1 in the Golgi apparatus with ab184014 at 1/500 dilution (green) and counterstained with Hoechst 33342. Cells were fixed in 100% methanol for 5 mins.
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Western blot - Anti-USO1 antibody (ab184014)All lanes : Anti-USO1 antibody (ab184014) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 108 kDa5% SDS-PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab184014 は 2 報の論文で使用されています。
- Zhang J et al. HDAC6 Inhibition Protects against OGDR-Induced Golgi Fragmentation and Apoptosis. Oxid Med Cell Longev 2019:6507537 (2019). PubMed: 31354911
- Zhong B et al. Hsp20 Protects against Oxygen-Glucose Deprivation/Reperfusion-Induced Golgi Fragmentation and Apoptosis through Fas/FasL Pathway. Oxid Med Cell Longev 2015:606934 (2015). WB . PubMed: 26199678