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AB252955

Anti-UROS 抗体

Anti-UROS antibody

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(1 Publication)

Rabbit Polyclonal UROS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human UROS aa 100-250.

別名を表示する

Uroporphyrinogen-III synthase, UROIIIS, UROS, Hydroxymethylbilane hydrolyase [cyclizing], Uroporphyrinogen-III cosynthase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UROS antibody (AB252955)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UROS antibody (AB252955)

Paraffin-embedded human colon tissue stained for UROS using ab252955 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-UROS antibody (AB252955)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UROS antibody (AB252955)

PFA-fixed, Triton X-100 permeabilized U-2OS (human brain glioma cell line) cells stained for UROS (green) using ab252955 at 4 μg/ml in ICC/IF.

Western blot - Anti-UROS antibody (AB252955)
  • WB

Supplier Data

Western blot - Anti-UROS antibody (AB252955)

All lanes:

Western blot - Anti-UROS antibody (ab252955) at 0.4 µg/mL

Lane 1:

Untransfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

HEK-293T transfected with UROS with a C-terminal myc-DDK tag, cell lysate.

Predicted band size: 28 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human UROS aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

P10746

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Uroporphyrinogen III synthase commonly known as UROS is an enzyme involved in heme biosynthesis. It catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III facilitating a ring closure and isomerization. UROS has a molecular mass of approximately 29 kDa. It is predominantly expressed in the liver bone marrow and erythroid tissues where heme production is essential. The enzyme is vital for maintaining proper levels of porphyrins in cells and ensuring the biosynthesis of heme an important component of hemoglobin.
Biological function summary

Uroporphyrinogen III synthase plays a role in heme production converting linear tetrapyrroles into the cyclic form needed for biosynthesis. This enzyme does not function as part of a larger complex but rather works independently to catalyze its specific reaction in porphyrin metabolism. Proper function of UROS is necessary to sustain the production of heme which is critical not only for oxygen transport in the blood but also for other heme-dependent processes in various tissues.

Pathways

UROS is an essential component of the heme biosynthesis pathway. In this pathway it acts between the steps that involve hydroxymethylbilane synthase (HMBS) and uroporphyrinogen III decarboxylase (UROD). This metabolic pathway is important for the production of heme which is a core component of hemoglobin. HMBS synthesizes the precursor transformed by UROS while UROD further modifies the product of UROS to continue the biosynthesis process emphasizing the enzyme's importance in maintaining the heme biosynthesis pathway's integrity.

Mutations or deficiencies in UROS are linked to a rare inherited condition called congenital erythropoietic porphyria (CEP) also known as Günther's disease. This disorder results from the accumulation of porphyrins owing to impaired heme biosynthesis. Abnormal function in UROS leads to a buildup of its substrates causing severe skin photosensitivity and hemolytic anemia among other symptoms. The functional relationship between UROS and HMBS or UROD in the heme biosynthesis pathway illustrates the enzyme's significant role in preventing the development of such heme-related disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, the branch point for the various sub-pathways leading to the wide diversity of porphyrins (PubMed : 11689424, PubMed : 18004775). Porphyrins act as cofactors for a multitude of enzymes that perform a variety of processes within the cell such as methionine synthesis (vitamin B12) or oxygen transport (heme) (PubMed : 11689424, PubMed : 18004775).
See full target information UROS
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6876 PubMed40715139

2025

The mechanotransducer Piezo1 coordinates metabolism and inflammation to promote skin growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchao Xue,Elizabeth Winnicki,Zhaoxu Zhang,Ines Lopez,Saifeng Wang,Charles Kirby,Sam S Lee,Ang Li,Chaewon Lee,Hana Minsky,Kaitlin Williams,Kevin Yueh-Hsun Yang,Ling He,Sashank K Reddy,Luis A Garza
View all publications
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