Anti-Uroplakin III 抗体 [EPR14420] (ab187646)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin III antibody [EPR14420] (ab187646)](https://www.abcam.co.jp/ps/products/187/ab187646/Images/ab187646-220634-anti-uroplakin-iii-antibody-epr14420-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin III antibody [EPR14420] (ab187646)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14420] to Uroplakin III
- Suitable for: IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Uroplakin III antibody [EPR14420]
Uroplakin III 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14420] to Uroplakin III -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human urothelium carcinoma tissue; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14420 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187646の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/32000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Flow Cyt (Intra) |
1/130.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
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IHC-P
1/32000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Flow Cyt (Intra)
1/130. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence. -
組織特異性
Expressed in ureter. -
関連疾患
Defects in UPK3A are a cause of renal adysplasia (RADYS) [MIM:191830]; also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy. -
配列類似性
Belongs to the uroplakin-3 family. -
細胞内局在
Endoplasmic reticulum membrane. Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER). - Information by UniProt
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参照データベース
- Entrez Gene: 7380 Human
- Omim: 611559 Human
- SwissProt: O75631 Human
- Unigene: 632787 Human
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別名
- MGC119178 antibody
- OTTHUMP00000028951 antibody
- UP III antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin III antibody [EPR14420] (ab187646)
Immunohistochemical analysis of paraffin-embedded Human urothelium carcinoma tissue labeling Uroplakin III with ab187646 at 1/32000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-Uroplakin III antibody [EPR14420] (ab187646)](https://www.abcam.co.jp/ps/products/187/ab187646/Images/ab187646-220635-anti-uroplakin-iii-antibody-epr14420-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Uroplakin III antibody [EPR14420] (ab187646)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedHeLa cells labeling Uroplakin III with ab187646 at 1/130 dilution compared to a Rabbit monoclonal IgG isotype control,followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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![Anti-Uroplakin III antibody [EPR14420] (ab187646)](https://www.abcam.co.jp/ps/products/187/ab187646/Images/ab187646-3-benefits-of-recombinant-antibodies.png)
Anti-Uroplakin III antibody [EPR14420] (ab187646)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab187646 は 1 報の論文で使用されています。
- Chen WY et al. IL-33/ST2 axis mediates hyperplasia of intrarenal urothelium in obstructive renal injury. Exp Mol Med 50:36 (2018). WB ; Mouse . PubMed: 29674622