Anti-Urokinase 抗体 (ab24121)
Key features and details
- Rabbit polyclonal to Urokinase
- Suitable for: IHC-Fr, ELISA, IHC-P, ICC/IF, Dot blot, RIA, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Urokinase antibody
Urokinase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Urokinase -
由来種
Rabbit -
特異性
This antibody binds to human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa). This antibody is highly specific for urokinase, no crossreactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human whole plasma or plasma proteins do not interfere with its binding to urokinase. -
アプリケーション
適用あり: IHC-Fr, ELISA, IHC-P, ICC/IF, Dot blot, RIA, WBmore details -
種交差性
交差種: Human -
免疫原
Full length protein (Human). Extensively purified native HMW-tcuPA and LMW-scuPA (mixture 50/50), isolated from human urine.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 8.00
Constituents: 0.16% Sodium phosphate, 0.38% Sodium borate, 0.87% Sodium chloride, 1% Dextran, 1% Mannitol -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is subjected to absorption with immobilized total human serum proteins to remove non-specific antibodies. The affinity purified antibody is obtained by binding to immobilized native human urokinase, followed by elution with acidic buffer, neutralization, dialysis, dispensing and lyophilization. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24121の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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RIA |
Use at an assay dependent concentration.
|
|
WB | (2) |
Use at an assay dependent concentration.
|
特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
RIA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
組織特異性
Expressed in the prostate gland and prostate cancers. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
翻訳後修飾
Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
- Unigene: 77274 Human
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別名
- ATF antibody
- ATF uPA antibody
- BDPLT5 antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab24121 は 18 報の論文で使用されています。
- Tan J et al. Proteomics analysis uncovers plasminogen activator PLAU as a target of the STING pathway for suppression of cancer cell migration and invasion. J Biol Chem 299:102779 (2023). PubMed: 36496076
- Hao L et al. CRISPR-Cas-amplified urinary biomarkers for multiplexed and portable cancer diagnostics. Nat Nanotechnol 18:798-807 (2023). PubMed: 37095220
- Zheng J et al. Deciphering intercellular signaling complexes by interaction-guided chemical proteomics. Nat Commun 14:4138 (2023). PubMed: 37438365
- Peng W et al. GLIPR1 Protects Against Cigarette Smoke-Induced Airway Inflammation via PLAU/EGFR Signaling. Int J Chron Obstruct Pulmon Dis 16:2817-2832 (2021). PubMed: 34675506
- Sundaram GM et al. HuR enhances FSTL1 transcript stability to promote invasion and metastasis of squamous cell carcinoma. Am J Cancer Res 11:4981-4993 (2021). PubMed: 34765305