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Anti-Urease 抗体 (ab34806)

Submit a review Q&A (1)References (2)

Key features and details

  • Rabbit polyclonal to Urease
  • Suitable for: ELISA
  • Reacts with: Canavalia ensiformis
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Urease antibody

  • 製品の詳細

    Rabbit polyclonal to Urease

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ELISAmore details

  • 種交差性

    交差種: Canavalia ensiformis

  • 免疫原

    Full length protein corresponding to Urease. Urease (Jack Bean)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Lyophilized:Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent).

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.164% Sodium phosphate
  • Concentration information loading...

  • 精製度

    Whole antiserum

  • 特記事項（精製）

    Anti-Urease (Jack Bean) (Rabbit) Antibody was prepared from monospecific antiserum by a delipidation and defibrination.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab34806の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
特記事項
ELISA
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    The enzyme urease catalyzes the hydrolysis of urea to create carbon dioxide and ammonia. The protein consists of a single kind of polypeptide chain containing 840 amino acid residues. The subunit relative molecular mass calculated from the sequence is 90,770, indicating that urease is composed of six subunits.

  • 細胞内局在

    Cytoplasmic

  • 別名

    • Urea Amidohydrolase antibody

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab34806 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab34806 は 2 報の論文で使用されています。

  • Jing W  et al. Metabolic Modulation of Intracellular Ammonia via Intravesical Instillation of Nanoporter-Encased Hydrogel Eradicates Bladder Carcinoma. Adv Sci (Weinh) 10:e2206893 (2023). PubMed: 36775865
  • Lian DW  et al. Effect of patchouli alcohol on macrophage mediated Helicobacter pylori digestion based on intracellular urease inhibition. Phytomedicine 65:153097 (2019). PubMed: 31568921

レビューと Q&A

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レビューを送る 質問を送る

Question

Dear technical support:
This customer is highly interested in ab34613 (Anti-Urease antibody (HRP)). This customer would likes to conduct the wb with Mycobacterium tuberculosis and bacterium sample, so she wants to know whether this antibody can work in this experiment or not? Could you please help this customer to solve the problem? Thank for your kindly assistance.
Best regards

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Answer

Thank you for contacting us and for your interest in our products.

As far as we are aware, ab34613 and our other two Urease antibodies ab34806 and ab34629 have never been tested with samples from Mycobacterium tuberculosis. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

I can confirm that the immunogen sequence for all 3 antibodies is taken from the Jack Bean Urease sequence which can be found on the following link:

http://www.uniprot.org/uniprot/P07374

I am sorry I am not able to find the Mycobacterium tuberculosis sequence on the protein databases. We recommend alignment should be 85% to predict that an antibody will detect in a different species. If you have the Mycobacterium tuberculosis sequence, I can recommend tocheck the alignment.

If the alignment is high,please do not hesitate to contact me agian prior to the purchase as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

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