Anti-UQCRFS1/RISP 抗体 [5A5] (ab14746)
Key features and details
- Mouse monoclonal [5A5] to UQCRFS1/RISP
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-UQCRFS1/RISP antibody [5A5]
UQCRFS1/RISP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5A5] to UQCRFS1/RISP -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cyt, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Cow, Human
交差が予測される動物種: Pig -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human, bovine, rat and murine heart mitochondria. In Flow Cytometry, this antibody gave a positive signal in Raji cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
5A5 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14746の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa.
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IHC-P | (1) |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa. |
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex. -
配列類似性
Contains 1 Rieske domain. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7386 Human
- Entrez Gene: 66694 Mouse
- Entrez Gene: 291103 Rat
- Omim: 191327 Human
- SwissProt: P13272 Cow
- SwissProt: P47985 Human
- SwissProt: Q9CR68 Mouse
- SwissProt: P20788 Rat
see all -
別名
- Complex III subunit 5 antibody
- Complex III subunit IX antibody
- Cytochrome b c1 complex subunit Rieske mitochondrial antibody
see all
画像
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All lanes : Anti-UQCRFS1/RISP antibody [5A5] (ab14746) at 0.5 µg/ml
Lane 1 : Hela Whole Cell Lysate - Hydroxyurea Treated
Lane 2 : Hela Whole Cell Lysate - Bleomycin Treated
Lane 3 : Hela Whole Cell Lysate - Staurosporine Treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Human normal kidney (cortex). Staining is observed intracellular. Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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All lanes : Anti-UQCRFS1/RISP antibody [5A5] (ab14746) at 1 µg/ml
Lane 1 : Isolated mitochondria from human heart at 5 µg
Lane 2 : Isolated mitochondria from bovine heart at 1 µg
Lane 3 : Isolated mitochondria from rat heart at 10 µg
Lane 4 : Isolated mitochondria from mouse heart at 10 µg -
Overlay histogram showing Raji cells stained with ab14746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14746, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (80)
ab14746 は 80 報の論文で使用されています。
- Moschandrea C et al. Mitochondrial dysfunction abrogates dietary lipid processing in enterocytes. Nature 625:385-392 (2024). PubMed: 38123683
- Kobayashi A et al. A FRET-based respirasome assembly screen identifies spleen tyrosine kinase as a target to improve muscle mitochondrial respiration and exercise performance in mice. Nat Commun 14:312 (2023). PubMed: 36697396
- Clements RT et al. Sexual dimorphism in bidirectional SR-mitochondria crosstalk in ventricular cardiomyocytes. Basic Res Cardiol 118:15 (2023). PubMed: 37138037
- Robinson DRL et al. Applying Sodium Carbonate Extraction Mass Spectrometry to Investigate Defects in the Mitochondrial Respiratory Chain. Front Cell Dev Biol 10:786268 (2022). PubMed: 35300415
- Santana-Codina N et al. NCOA4-Mediated Ferritinophagy Is a Pancreatic Cancer Dependency via Maintenance of Iron Bioavailability for Iron-Sulfur Cluster Proteins. Cancer Discov 12:2180-2197 (2022). PubMed: 35771492