AB169754

Anti-uPA 抗体 [EP6274(2)]

Name: Anti-uPA 抗体 [EP6274(2)] (ab169754) | Abcam
Brand: Abcam Limited
SKU: AB169754
Price: 29000 JPY
Availability: InStock
Rating: 1 (1 reviews)

Anti-uPA antibody [EP6274(2)]

20ul selling size
RabMAb
Recombinant
詳細を見る

(1 Review)

(14 Publications)

Rabbit Recombinant Monoclonal Urokinase antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 14 publications.

別名を表示する

Urokinase-type plasminogen activator, U-plasminogen activator, uPA, PLAU

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-uPA antibody [EP6274(2)] (AB169754)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-uPA antibody [EP6274(2)] (AB169754)

Immunocytochemistry analysis of PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling uPA with Purified ab169754 at 1 : 50 dilution (3.78 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Unknown

Western blot - Anti-uPA antibody [EP6274(2)] (AB169754)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-uPA antibody [EP6274(2)] (ab169754) at 1/1000 dilution

All lanes:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 49 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP6274(2)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Chimpanzee": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

See full target information Urokinase-type plasminogen activator

style

left-column

文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:835299 PubMed35707350

2022

Hypermethylation of the Promoter Region of miR-23 Enhances the Metastasis and Proliferation of Multiple Myeloma Cells the Aberrant Expression of uPA.

Applications

Unspecified application

Species

Unspecified reactive species

Qijie Ran,Dehong Xu,Qi Wang,Dongsheng Wang

Cancers 14: PubMed35053621

2022

Molecular Landscape of the Coagulome of Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Marine Lottin,Simon Soudet,Julie Fercot,Floriane Racine,Julien Demagny,Jérémie Bettoni,Denis Chatelain,Marie-Antoinette Sevestre,Youcef Mammeri,Michele Lamuraglia,Antoine Galmiche,Zuzana Saidak

Genes 12: PubMed34828412

2021

Two Rare Variants in and Genes-Do They Contribute to Semantic Dementia Clinical Phenotype?

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Gaweda-Walerych,Emilia J Sitek,Małgorzata Borczyk,Mariusz Berdyński,Ewa Narożańska,Bogna Brockhuis,Michał Korostyński,Jarosław Sławek,Cezary Zekanowski

Bioengineered 12:9753-9765 PubMed34696681

2021

Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Sun,Fang Zhou,Juan Xue,Chunyan Ji,Yinzong Qu,Yuwei Pan

Cell death discovery 7:32 PubMed33574243

2021

PLAU directs conversion of fibroblasts to inflammatory cancer-associated fibroblasts, promoting esophageal squamous cell carcinoma progression via uPAR/Akt/NF-κB/IL8 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Fang,Yun Che,Chaoqi Zhang,Jianbing Huang,Yuanyuan Lei,Zhiliang Lu,Nan Sun,Jie He

Pharmacological research 143:73-85 PubMed30862605

2019

Novel urokinase-plasminogen activator inhibitor SPINK13 inhibits growth and metastasis of hepatocellular carcinoma in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wei,Yongzhi Lun,Xiaoping Zhou,Shang He,Lijuan Gao,Yan Liu,Zheng He,Baoming Li,Chengbin Wang

OncoTargets and therapy 11:7733-7743 PubMed30464522

2018

MicroRNA-645 targets urokinase plasminogen activator and decreases the invasive growth of MDA-MB-231 triple-negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Du Meng,Ming Lei,Yaxuan Han,Dongli Zhao,Xiaozhi Zhang,Yunyi Yang,Rui Liu

Oncology research 27:515-524 PubMed29386086

2018

Upregulation of miR-324-5p Inhibits Proliferation and Invasion of Colorectal Cancer Cells by Targeting ELAVL1.

Applications

Unspecified application

Species

Unspecified reactive species

Chijiang Gu,Mingyuan Zhang,Weiliang Sun,Changzheng Dong

Scientific reports 7:13570 PubMed29051577

2017

De novo mutations of TUBA3D are associated with keratoconus.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Dan Hao,Peng Chen,Yang-Yang Zhang,Su-Xia Li,Wei-Yun Shi,Hua Gao

Oncology letters 14:7288-7296 PubMed29344165

2017

Overexpression of forkhead box M1 and urokinase-type plasminogen activator in gastric cancer is associated with cancer progression and poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ma,Guangwei Qi,Ji Xu,Haibing Ni,Wulin Xu,Guoqing Ru,Zhongsheng Zhao,Wenjuan Xu,Xujun He

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-uPA 抗体 [EP6274(2)]

Anti-uPA antibody [EP6274(2)]

Immunocytochemistry/ Immunofluorescence - Anti-uPA antibody [EP6274(2)] (AB169754)

Western blot - Anti-uPA antibody [EP6274(2)] (AB169754)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (14)

Abcam product promise