Anti-UNG 抗体 (ab47680)
Key features and details
- Rabbit polyclonal to UNG
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-UNG antibody
UNG 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UNG -
由来種
Rabbit -
特異性
This antibody is specific to UNG1. It will not cross react with UNG2. -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
A 13 amino acid peptide from near the amino terminus of human UNG1
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ポジティブ･コントロール
- C2C12 cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47680の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use a concentration of 2 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa). |
|
ICC/IF
Use a concentration of 20 µg/ml. |
|
IHC-P
Use a concentration of 2 µg/ml. |
ターゲット情報
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機能
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. -
組織特異性
Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells. -
関連疾患
Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step. -
配列類似性
Belongs to the uracil-DNA glycosylase family. -
翻訳後修飾
Isoform 1 is processed by cleavage of a transit peptide. -
細胞内局在
Mitochondrion and Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 7374 Human
- Entrez Gene: 22256 Mouse
- Omim: 191525 Human
- SwissProt: P13051 Human
- SwissProt: P97931 Mouse
- Unigene: 191334 Human
- Unigene: 1393 Mouse
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別名
- DGU antibody
- DKFZp781L1143 antibody
- HIGM 4 antibody
see all
画像
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Western blot - Anti-UNG antibody (ab47680)Lane 1 : Anti-UNG antibody (ab47680) at 0.5 µg/ml
Lane 2 : Anti-UNG antibody (ab47680) at 1 µg/ml
Lane 3 : Anti-UNG antibody (ab47680) at 2 µg/ml
All lanes : C2C12 lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 35 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Anti rabbit IgG secondary antibodies can be used. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG antibody (ab47680)ab37170 at 2 µg/ml staining UNG in human heart tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
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Immunocytochemistry/ Immunofluorescence - Anti-UNG antibody (ab47680)Immunofluorescence of UNG1 in Human Heart cells using ab47680 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab47680 は 1 報の論文で使用されています。
- Ferrando B et al. DNA repair in plant mitochondria - a complete base excision repair pathway in potato tuber mitochondria. Physiol Plant 166:494-512 (2019). PubMed: 30035320