Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-UNC5C 抗体 [EPR24190-37] - BSA and Azide free (ab302925)

Submit a review Submit a question
Western blot - Anti-UNC5C antibody [EPR24190-37] - BSA and Azide free (ab302925)
  • Anti-UNC5C antibody [EPR24190-37] (BSA and Azide free) (AB302925)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24190-37] to UNC5C - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-UNC5C antibody [EPR24190-37] - BSA and Azide free
    UNC5C 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24190-37] to UNC5C - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse brain, cerebral cortex, hippocampus and hypothalamus tissue lysate. Rat brain tissue lysate. SH-SY5Y whole cell lysate.

  • 特記事項

    AB302925 is a carrier free version of AB302924. 

    AB302924 does not react in: IHC-P, ICC/IF, Flow Cytometry (Intra) or IP with human, mouse and rat samples. 

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302925の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 130, 66 kDa (predicted molecular weight: 103 kDa).
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 130, 66 kDa (predicted molecular weight: 103 kDa).
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. Also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.

  • 組織特異性

    Mainly expressed in brain. Also expressed in kidney. Not expressed in developing or adult lung.

  • 配列類似性

    Belongs to the unc-5 family.
    Contains 1 death domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 2 TSP type-1 domains.
    Contains 1 ZU5 domain.

  • 翻訳後修飾

    Phosphorylated on different cytoplasmic tyrosine residues. Phosphorylation of Tyr-568 leads to an interaction with PTPN11 phosphatase, suggesting that its activity is regulated by phosphorylation/dephosphorylation. Tyrosine phosphorylation is netrin-dependent.
    Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • homolog of C. elegans transmembrane receptor Unc5 antibody
    • Netrin receptor UNC5C antibody
    • Protein unc 5 homolog C antibody
    • Protein unc-5 homolog 3 antibody
    • Protein unc-5 homolog C antibody
    • Unc 5 homolog 3 antibody
    • Unc 5 homolog C antibody
    • Unc5 (C.elegans homolog) c antibody
    • Unc5c antibody
    • UNC5C_HUMAN antibody
    • UNC5H3 antibody
    see all

画像

  • Western blot - Anti-UNC5C antibody [EPR24190-37] - BSA and Azide free (ab302925)
    Western blot - Anti-UNC5C antibody [EPR24190-37] - BSA and Azide free (ab302925)
    All lanes : Anti-UNC5C antibody [EPR24190-37] (ab302924) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse cerebral cortex tissue lysate
    Lane 3 : Mouse hippocampus tissue lysate
    Lane 4 : Mouse hypothalamus tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
    Lane 7 : 293T (human embryonic kidney epithelial cell), whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 103 kDa
    Observed band size: 130, 66 kDa why is the actual band size different from the predicted?



    This data was developed using AB302924, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST

    Negative control: 293T(PMID: 21893118)

    The molecular weight observed is consistent with what has been described in the literature (PMID: 27716118; 33863723).

    This blot was developed using a high sensitivity ECL substrate in lane 5. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    Samples are non-boiled as boiling may cause protein aggregates. 

    Exposure time: 

    Lane 1-4, 6-7: 180 seconds

    Lane 5: 37 seconds 

  • Anti-UNC5C antibody [EPR24190-37] (BSA and Azide free) (AB302925)
    Anti-UNC5C antibody [EPR24190-37] (BSA and Azide free) (AB302925)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab302925 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302925 は論文での使用が確認できていません。

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

There are currently no Customer reviews or Questions for ab302925.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com