Anti-UNC5B 抗体 [Guido-1] (ab54430)
![Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (ab54430)](https://www.abcam.co.jp/ps/products/54/ab54430/Images/ab54430-67279-anti-unc5b-antibody-guido-1-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (ab54430)")
Key features and details
- Mouse monoclonal [Guido-1] to UNC5B
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-UNC5B antibody [Guido-1]
UNC5B 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [Guido-1] to UNC5B -
由来種
Mouse -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to amino acids 28-363 of Human UNC5B
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特記事項
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
Guido-1 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab54430の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Functions as netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (By similarity). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed:12598906). Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity. -
組織特異性
Highly expressed in brain. Also expressed at lower level in developing lung, cartilage, kidney and hematopoietic and immune tissues. -
配列類似性
Belongs to the unc-5 family.
Contains 1 death domain.
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 2 TSP type-1 domains.
Contains 1 ZU5 domain. -
翻訳後修飾
Phosphorylated on cytoplasmic tyrosine residues.
Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
Palmitoylation is required for pro-apoptotic activity, but not for location at lipid rafts. -
細胞内局在
Cell membrane. Membrane raft. Associated with lipid rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 219699 Human
- Omim: 607870 Human
- SwissProt: Q8IZJ1 Human
- Unigene: 522997 Human
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別名
- 6330415E02Rik antibody
- A630020F16 antibody
- D10Bwg0792e antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (ab54430)
ICC/IF image of ab54430 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54430, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab54430 は 5 報の論文で使用されています。
- Huang Y et al. The intracellular domain of UNC5B facilities proliferation and metastasis of bladder cancer cells. J Cell Mol Med 25:2121-2135 (2021). PubMed: 33345442
- Srikanth P et al. Convergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression. Transl Psychiatry 8:245 (2018). PubMed: 30410030
- Miloudi K et al. Truncated netrin-1 contributes to pathological vascular permeability in diabetic retinopathy. J Clin Invest 126:3006-22 (2016). PubMed: 27400127
- Ramkhelawon B et al. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat Med 20:377-84 (2014). IF . PubMed: 24584118
- König K et al. The uncoordinated-5 homolog B receptor affects hepatic ischemia reperfusion injury. PLoS One 7:e41085 (2012). Flow Cyt ; Mouse . PubMed: 22848430