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AB324867

Anti-UNC119-binding protein C5orf30 antibody [EPR30038-27]

Anti-UNC119-binding protein C5orf30 antibody [EPR30038-27]

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Rabbit Recombinant Monoclonal UNC119-binding protein C5orf30 antibody. Suitable for WB and reacts with Human samples.

別名を表示する

C5orf30, MACIR, Macrophage immunometabolism regulator

2 Images
Western blot - Anti-UNC119-binding protein C5orf30 antibody [EPR30038-27] (AB324867)
  • WB

Supplier Data

Western blot - Anti-UNC119-binding protein C5orf30 antibody [EPR30038-27] (AB324867)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-UNC119-binding protein C5orf30 antibody [EPR30038-27] (ab324867) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting UNC119-binding protein C5orf30 whole cell lysate at 20 µg

Lane 3:

Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 23 kDa,36 kDa

false

Exposure time: 26s

Western blot - Anti-UNC119-binding protein C5orf30 antibody [EPR30038-27] (AB324867)
  • WB

Supplier Data

Western blot - Anti-UNC119-binding protein C5orf30 antibody [EPR30038-27] (AB324867)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Daudi, Jurkat (PMID : 26316022).

The identity of the bands higher than 37 kDa and lower than 20 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-UNC119-binding protein C5orf30 antibody [EPR30038-27] (ab324867) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Daudi (human burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 3:

Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 20 µg

Lane 4:

HT-1376 (human urinary bladder carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

HDLM-2 (human hodgkin lymphoma cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 23 kDa,36 kDa

false

Exposure time: 26s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR30038-27

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Regulates the macrophage function, by enhancing the resolution of inflammation and wound repair functions mediated by M2 macrophages (PubMed : 30659109). The regulation of macrophage function is, due at least in part, to its ability to inhibit glycolysis (PubMed : 30659109). May also play a role in trafficking of proteins via its interaction with UNC119 and UNC119B cargo adapters : may help the release of UNC119 and UNC119B cargo or the recycling of UNC119 and UNC119B (PubMed : 22085962). May play a role in ciliary membrane localization via its interaction with UNC119B and protein transport into photoreceptor cells (PubMed : 22085962).
See full target information MACIR
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Abcam product promise

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