Anti-UMOD 抗体 (ab167678)
Key features and details
- Mouse polyclonal to UMOD
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-UMOD antibody
UMOD 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to UMOD -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human UMOD aa 1-650. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (UniProt ID: P07911-3; AAH35975)
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ポジティブ･コントロール
- UMOD transfected 293T cell lysate; Human kidney tissue; HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167678の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa.
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| IHC-P | (1) |
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. |
|
IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Not known. May play a role in regulating the circulating activity of cytokines as it binds to IL-1, IL-2 and TNF with high affinity. -
組織特異性
Synthesized by kidney. Most abundant protein in normal human urine. -
関連疾患
Defects in UMOD are the cause of familial juvenile hyperuricemic nephropathy type 1 (HNFJ1) [MIM:162000]. HNFJ1 is a renal disease characterized by juvenil onset of hyperuricemia, polyuria, progressive renal failure, and gout. The disease is associated with interstitial pathological changes resulting in fibrosis.
Defects in UMOD are the cause of medullary cystic kidney disease type 2 (MCKD2) [MIM:603860]. MCKD2 is a form of tubulointerstitial nephropathy characterized by formation of renal cysts at the corticomedullary junction. It is characterized by adult onset of impaired renal function and salt wasting resulting in end-stage renal failure by the sixth decade.
Defects in UMOD are the cause of glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI) [MIM:609886]. GCKDHI is a renal disorder characterized by a cystic dilation of Bowman space, a collapse of glomerular tuft, and hyperuricemia due to low fractional excretion of uric acid and severe impairment of urine concentrating ability. -
配列類似性
Contains 3 EGF-like domains.
Contains 1 ZP domain. -
細胞内局在
Cell membrane. Secreted. Secreted after cleavage in the urine. - Information by UniProt
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参照データベース
- Entrez Gene: 7369 Human
- Omim: 191845 Human
- SwissProt: P07911 Human
- Unigene: 654425 Human
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別名
- ADMCKD2 antibody
- FJHN antibody
- HNFJ antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-UMOD antibody (ab167678)
Immunofluorescence analysis of HeLa cells, labeling UMOD with ab167678 at 10 µg/ml.
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Western blot - Anti-UMOD antibody (ab167678)All lanes : Anti-UMOD antibody (ab167678) at 1 µg/ml
Lane 1 : UMOD transfected 293T cell lysate
Lane 2 : Non-transfected transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 67 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody (ab167678)Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling UMOD with ab167678 at 3 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab167678 は論文での使用が確認できていません。
