Anti-ULK1 抗体 (ab167139)
Key features and details
- Rabbit polyclonal to ULK1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-ULK1 antibody
ULK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ULK1 -
由来種
Rabbit -
特異性
Two alternatively spliced transcript variants encoding different isoforms have been identified. ab167139 is predicted to not cross-react with ULK2. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide, corresponding to 16 internal sequence amino acids of Human ULK1 (NP_003556).
-
ポジティブ･コントロール
- WB: Rat brain and testis tissue lysate, mouse lung, brain, thymus and pancreas, HepG2, MCF-7.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167139の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa. |
ターゲット情報
-
機能
Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165). -
組織特異性
Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy. -
細胞内局在
Cytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. - Information by UniProt
-
参照データベース
- Entrez Gene: 8408 Human
- Entrez Gene: 22241 Mouse
- Entrez Gene: 360827 Rat
- Omim: 603168 Human
- SwissProt: O75385 Human
- SwissProt: O70405 Mouse
- Unigene: 47061 Human
- Unigene: 271898 Mouse
-
別名
- ATG 1 antibody
- ATG1 antibody
- ATG1 autophagy related 1 homolog antibody
see all
画像
-
Western blot - Anti-ULK1 antibody (ab167139)All lanes : Anti-ULK1 antibody (ab167139) at 2 µg/ml
Lane 1 : Rat brain lysate
Lane 2 : Rat testis lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 112 kDa
Exposure time: 15 minutes1 h incubation at RT in 5% NFDM/TBST
-
Western blot - Anti-ULK1 antibody (ab167139)All lanes : Anti-ULK1 antibody (ab167139) at 2 µg/ml
Lane 1 : HepG2 lysate
Lane 2 : MCF-7 lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 112 kDa
Exposure time: 5 minutes1 h incubation at RT in 5% NFDM/TBST
-
Western blot - Anti-ULK1 antibody (ab167139)All lanes : Anti-ULK1 antibody (ab167139) at 2 µg/ml
Lane 1 : Mouse lung at 15 µg
Lane 2 : Mouse brain at 15 µg
Lane 3 : Mouse thymus at 15 µg
Lane 4 : Mouse pancreas
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 112 kDa
Exposure time: 5 minutes1 h incubation at RT in 5% NFDM/TBST
-
Western blot - Anti-ULK1 antibody (ab167139)All lanes : Anti-ULK1 antibody (ab167139) at 1 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat brain tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 112 kDa
データシートおよび資料
-
Datasheet download
参考文献 (20)
ab167139 は 20 報の論文で使用されています。
- Cai C et al. Empagliflozin attenuates cardiac microvascular ischemia/reperfusion through activating the AMPKα1/ULK1/FUNDC1/mitophagy pathway. Redox Biol 52:102288 (2022). PubMed: 35325804
- Wu M et al. The Evaluation of Xiaozeng Qianggu Tablets for Treating Postmenopausal Osteoporosis via up-Regulated Autophagy. Evid Based Complement Alternat Med 2022:3960834 (2022). PubMed: 36193128
- Zheng Y et al. miR-1262 suppresses gastric cardia adenocarcinoma via targeting oncogene ULK1. J Cancer 12:1231-1239 (2021). PubMed: 33442421
- Zhang J et al. PSPH induces cell autophagy and promotes cell proliferation and invasion in the hepatocellular carcinoma cell line Huh7 via the AMPK/mTOR/ULK1 signaling pathway. Cell Biol Int 45:305-319 (2021). PubMed: 33079432
- Sun X et al. Activation of Autophagy Through the NLRP3/mTOR Pathway: A Potential Mechanism for Alleviation of Pneumonia by QingFei Yin. Front Pharmacol 12:763160 (2021). PubMed: 35111047
