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リコンビナントRabMAb

Anti-ULBP3 抗体 [EPR22321-99] (ab300102)

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Western blot - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
  • Western blot - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
  • Western blot - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
  • Flow Cytometry - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
  • Flow Cytometry - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
  • Immunoprecipitation - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
  • Dot Blot - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
  • Anti-ULBP3 antibody [EPR22321-99] (AB300102)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22321-99] to ULBP3
  • Suitable for: Dot blot, Flow Cyt, WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ULBP3 antibody [EPR22321-99]
    ULBP3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22321-99] to ULBP3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Dot blot, Flow Cyt, WB, IPmore details
    適用なし: ICC/IF or IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Whole cell lysates: SW480 (human colorectal adenocarcinoma epithelial cell), MDA-MB-231 (human breast adenocarcinoma epithelial cell), HeLa, MCF7 (human breast adenocarcinoma epithelial cell), NAMALWA (human burkitt's lymphoma b lymphocyte), Raji (human Burkitt's lymphoma b lymphocyte). Human testis tissue lysate. Flow cyt.: MCF7, Hela, Raji, NAMALWA. IP: MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate. DB: Human ULBP3 recombinant protein

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR22321-99

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300102の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Dot blot
1/1000.
Flow Cyt
1/500.
WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
IP
1/30.
特記事項
Dot blot
1/1000.
Flow Cyt
1/500.
WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
IP
1/30.
追加情報
Is unsuitable for ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Ligand for the KLRK1/NKG2D receptor, together with at least ULBP1 and ULBP2. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to KLRK1/NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. Has lower affinity for KLRK1/NKG2D compared to ULBP1 and ULBP2 and induces weaker signaling responses than does ULBP2 or ULBP1.

  • 配列類似性

    Belongs to the MHC class I family.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ALCAN- gamma antibody
      • ALCAN-gamma antibody
      • N2DL-3 antibody
      • N2DL3 antibody
      • N2DL3_HUMAN antibody
      • NKG2D ligand 3 antibody
      • NKG2D ligand 3 precursor antibody
      • NKG2DL3 antibody
      • OTTHUMP00000017411 antibody
      • RAET1N antibody
      • Retinoic acid early transcript 1N antibody
      • UL16 binding protein 3 antibody
      • UL16-binding protein 3 antibody
      • ULBP3 antibody
      see all

    画像

    • Western blot - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
      Western blot - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
      All lanes : Anti-ULBP3 antibody [EPR22321-99] (ab300102) at 1/1000 dilution

      Lane 1 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
      Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 28 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Exposure time: 3 minutes. 

      Low expression cell line: MCF7(PMID: 25217158).

    • Western blot - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
      Western blot - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
      All lanes : Anti-ULBP3 antibody [EPR22321-99] (ab300102) at 1/1000 dilution

      Lane 1 : NAMALWA (human burkitt's lymphoma b lymphocyte) whole cell lysate
      Lane 2 : Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 28 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Exposure time: 3 minutes. 

      Low expression: Raji (PMID: 12414645, 11827464).

    • Western blot - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
      Western blot - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
      Anti-ULBP3 antibody [EPR22321-99] (ab300102) at 1/1000 dilution + Human testis tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 28 kDa


      Exposure time: 3 minutes


      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes. 

    • Flow Cytometry - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
      Flow Cytometry - Anti-ULBP3 antibody [EPR22321-99] (AB300102)

      Flow cytometric analysis of Raji (human burkitt's lymphoma b lymphocyte, Left) / NAMALWA (human burkitt's lymphoma b lymphocyte, Right) labeling ULBP3 with ab300102 at 1/500 dilution (Red) compared to isotope control Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/2000 dilution was used as secondary antibody. 

      Gated on viable cells.
      Low expression: Raji (PMID: 12414645, 11827464).

    • Flow Cytometry - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
      Flow Cytometry - Anti-ULBP3 antibody [EPR22321-99] (AB300102)

      Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / HeLa (human cervix adenocarcinoma epithelial cell, Right) labeling ULBP3 with ab300102 at 1/500 dilution (Red) compared to isotope control Rabbit monoclonal IgG (ab172730) (Black)  and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/2000 dilution was used as secondary antibody. 

      Gated on viable cells.
      Low expression: MCF7 (PMID: 25217158).

    • Immunoprecipitation - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
      Immunoprecipitation - Anti-ULBP3 antibody [EPR22321-99] (AB300102)

      ULBP3 was immunoprecipitated from MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg with ab300102 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300102 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg (Inset)

      Lane 2: ab300102 IP in MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300102 in MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 119 seconds. 

    • Dot Blot - Anti-ULBP3 antibody [EPR22321-99] (AB300102)
      Dot Blot - Anti-ULBP3 antibody [EPR22321-99] (AB300102)

      Dot blot analysis of ULBP3 using ab300102 at 1:1000 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

      Lane 1: Human ULBP3 recombinant protein 
      Lane 2: Human ULBP1 recombinant protein 
      Lane 3: Human ULBP2 recombinant protein 
      Lane 4: Human ULBP5 recombinant protein 
      Lane 5: Human ULBP6 recombinant protein 

      Exposure time: 3 minutes

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      No cross-reactivity with other family members.

    • Anti-ULBP3 antibody [EPR22321-99] (AB300102)
      Anti-ULBP3 antibody [EPR22321-99] (AB300102)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab300102 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300102 は 1 報の論文で使用されています。

    • Zhang KJ  et al. LncRNA TYMSOS facilitates breast cancer metastasis and immune escape through downregulating ULBP3. iScience 26:107556 (2023). PubMed: 37664624

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