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リコンビナントRabMAb

Anti-ULBP2+ULBP5+ULBP6 抗体 [EPR26171-156] (ab290731)

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Flow Cytometry - Anti-ULBP2 antibody [EPR26171-156] (ab290731)
  • Flow Cytometry (Intracellular) - Anti-ULBP2 antibody [EPR26171-156] (ab290731)
  • Flow Cytometry (Intracellular) - Anti-ULBP2 antibody [EPR26171-156] (ab290731)
  • Anti-ULBP2 antibody [EPR26171-156] (ab290731)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26171-156] to ULBP2
  • Suitable for: Flow Cyt (Intra), Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-ULBP2+ULBP5+ULBP6 antibody [EPR26171-156]
    ULBP2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26171-156] to ULBP2

  • 由来種

    Rabbit

  • 特異性

    IHC not suitable for human species.

  • アプリケーション

    適用あり: Flow Cyt (Intra), Flow Cytmore details
    適用なし: ICC/IF,IHC-P,IP or WB

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Flow Cyt (Intracellular): BxPC-3 cells and HEK-293T cells transfected with a human ULBP2, ULBP-5, and ULBP-6 expression vector. Flow Cyt: BxPC-3 cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR26171-156

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290731の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/500.
Flow Cyt
1/500.
特記事項
Flow Cyt (Intra)
1/500.
Flow Cyt
1/500.
追加情報
Is unsuitable for ICC/IF,IHC-P,IP or WB.

ターゲット情報

  • 機能

    Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface.

  • 組織特異性

    Expressed in various types of cancer cell lines and in the fetus, but not in normal tissues.

  • 配列類似性

    Belongs to the MHC class I family.

  • 細胞内局在

    Cell membrane. Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ALCAN alpha antibody
      • ALCAN-alpha antibody
      • N2DL 2 antibody
      • N2DL-2 antibody
      • N2DL2 antibody
      • N2DL2_HUMAN antibody
      • NKG2D ligand 2 antibody
      • NKG2D ligand 2 precursor antibody
      • NKG2DL2 antibody
      • RAET1H antibody
      • Retinoic acid early transcript 1 H antibody
      • Retinoic acid early transcript 1H antibody
      • UL16 binding protein 2 antibody
      • UL16-binding protein 2 antibody
      • ULBP2 antibody
      see all

    画像

    • Flow Cytometry - Anti-ULBP2 antibody [EPR26171-156] (ab290731)
      Flow Cytometry - Anti-ULBP2 antibody [EPR26171-156] (ab290731)

      Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte, Left) / BxPC-3 (Human pancreas adenocarcinoma epithelial cell, Right) labeling ULBP2 (red) with ab290731 at a 1/500 dilution. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody at a 1/2000 dilution. Black - Isotype control: Rabbit monoclonal IgG (ab172730). Blue - unlabeled control: Cells without incubation with primary and secondary antibodies.

      Negative control: Raji.

      Gated on viable cells.

    • Flow Cytometry (Intracellular) - Anti-ULBP2 antibody [EPR26171-156] (ab290731)
      Flow Cytometry (Intracellular) - Anti-ULBP2 antibody [EPR26171-156] (ab290731)

      Intracellular Flow Cytometry analysis of HEK-293T (Human embryonic kidney epithelial cell) transfected with a human ULBP2 expression vector labeling ULBP2 with ab290731 at a 1/500 dilution (Right). Cells were fixed with 2% paraformaldehyde and permeabilized with 0.1% Tween-20. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody at a 1/2000 dilution. Isotype control: Rabbit monoclonal IgG (ab172730) (Left).

      Cells were surface stained with rabbit IgG or ab290731, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 647.

    • Flow Cytometry (Intracellular) - Anti-ULBP2 antibody [EPR26171-156] (ab290731)
      Flow Cytometry (Intracellular) - Anti-ULBP2 antibody [EPR26171-156] (ab290731)

      Intracellular Flow Cytometry analysis of HEK-293T (Human embryonic kidney epithelial cell) transfected with human ULBP-5 (Left) and ULBP-6 (Right) expression vector labeling ULBP2 with ab290731 at a 1/500 dilution. Cells were fixed with 2% paraformaldehyde and permeabilized with 0.1% Tween-20. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody at a 1/2000 dilution.

      Cells were surface stained with ab290731, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 647.

    • Anti-ULBP2 antibody [EPR26171-156] (ab290731)
      Anti-ULBP2 antibody [EPR26171-156] (ab290731)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab290731 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290731 は論文での使用が確認できていません。

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