Anti-UGDH 抗体 (ab155005)
Key features and details
- Rabbit polyclonal to UGDH
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-UGDH antibody
UGDH 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UGDH -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human UGDH aa 9-283. The exact sequence is proprietary.
Database link: O60701 -
ポジティブ･コントロール
- WB: HepG2 whole cell lysate (ab7900) and mouse and rat liver tissue lysate. IHC-P: Human hepatoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155005の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
|
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate. -
組織特異性
Widely expressed. -
パスウェイ
Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1. -
配列類似性
Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. - Information by UniProt
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参照データベース
- Entrez Gene: 7358 Human
- Entrez Gene: 22235 Mouse
- Entrez Gene: 83472 Rat
- Omim: 603370 Human
- SwissProt: O60701 Human
- SwissProt: O70475 Mouse
- SwissProt: O70199 Rat
- Unigene: 572518 Human
see all -
別名
- GDH antibody
- UDP Glc dehydrogenase antibody
- UDP GlcDH antibody
see all
画像
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Western blot - Anti-UGDH antibody (ab155005)Anti-UGDH antibody (ab155005) at 1/10000 dilution + mouse liver whole cell lysate at 50 µg
Predicted band size: 55 kDa
7.5% SDS PAGE -
Western blot - Anti-UGDH antibody (ab155005)Anti-UGDH antibody (ab155005) at 1/2000 dilution + rat liver lysate/extract at 50 µg
Predicted band size: 55 kDa
7.5 % SDS-PAGE -
Western blot - Anti-UGDH antibody (ab155005)Anti-UGDH antibody (ab155005) at 1/10000 dilution + HepG2 whole cell lysate/extract at 30 µg
Predicted band size: 55 kDa
7.5 % SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (ab155005)Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labelling UGDH with ab155005 at a 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab155005 は 4 報の論文で使用されています。
- Sánchez-Martín P et al. NBR1-mediated p62-liquid droplets enhance the Keap1-Nrf2 system. EMBO Rep 21:e48902 (2020). PubMed: 31916398
- Szél E et al. Comprehensive Proteomic Analysis Reveals Intermediate Stage of Non-Lesional Psoriatic Skin and Points out the Importance of Proteins Outside this Trend. Sci Rep 9:11382 (2019). PubMed: 31388062
- Kageyama S et al. Negative Regulation of the Keap1-Nrf2 Pathway by a p62/Sqstm1 Splicing Variant. Mol Cell Biol 38:N/A (2018). PubMed: 29339380
- Saito T et al. p62/Sqstm1 promotes malignancy of HCV-positive hepatocellular carcinoma through Nrf2-dependent metabolic reprogramming. Nat Commun 7:12030 (2016). WB . PubMed: 27345495