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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-UFSP2 抗体 [EPR13424] (ab185965)

Submit a review Submit a question References (6)
Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)
  • Immunocytochemistry/ Immunofluorescence - Anti-UFSP2 antibody [EPR13424] (ab185965)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)
  • Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)
  • Immunoprecipitation - Anti-UFSP2 antibody [EPR13424] (ab185965)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)
  • Anti-UFSP2 antibody [EPR13424] (ab185965)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13424] to UFSP2
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-UFSP2 antibody [EPR13424]
    UFSP2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13424] to UFSP2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human fetal kidney, fetal heart, pancreas and HeLa cell lysates; HEK293T and HeLa cell lysates. IHC-P: Human stomach and kidney clear cell carcinoma tissues. ICC/IF: HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13424

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185965の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
IP
1/40 - 1/60.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
IP
1/40 - 1/60.

ターゲット情報

  • 機能

    Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of UFM1, a ubiquitin-like modifier protein bound to a number of target proteins. Does not hydrolyze SUMO1 or ISG15 ubiquitin-like proteins. Through TRIP4 deufmylation may regulate intracellular nuclear receptors transactivation and thereby regulate cell proliferation and differentiation.

  • 関連疾患

    Beukes familial hip dysplasia

  • 配列類似性

    Belongs to the peptidase C78 family.

  • 細胞内局在

    Cytoplasm. Endoplasmic reticulum. Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • C4orf20 antibody
    • Chromosome 4 open reading frame 20 antibody
    • FLJ11200 antibody
    • Ufm1 specific protease 2 antibody
    • UFM1-specific peptidase 2 antibody
    • Ufm1-specific protease 2 antibody
    • UfSP2 antibody
    • UFSP2_HUMAN antibody
    see all

画像

  • Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)
    Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)
    All lanes : Anti-UFSP2 antibody [EPR13424] (ab185965) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : UFSP2 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab185965 observed at 53 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab185965 Anti-UFSP2 antibody [EPR13424]  was shown to specifically react with UFSP2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266368 (knockout cell lysate ab257782) was used. Wild-type and UFSP2 knockout samples were subjected to SDS-PAGE. ab185965 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-UFSP2 antibody [EPR13424] (ab185965)
    Immunocytochemistry/ Immunofluorescence - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Immunofluorescent analysis of HeLa cells (-20℃ acetone-fixed) labeling UFSP2 with ab185965 at 1/100 dilution follwed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling UFSP2 with ab185965 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)
    Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)
    All lanes : Anti-UFSP2 antibody [EPR13424] (ab185965) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human pancreas lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa

  • Immunoprecipitation - Anti-UFSP2 antibody [EPR13424] (ab185965)
    Immunoprecipitation - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Western blot analysis of immunoprecipitation pellet from Human fetal kidney lysate immunoprecipitated using ab185965 at 1/50 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling UFSP2 with ab185965 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-UFSP2 antibody [EPR13424] (ab185965)
    Anti-UFSP2 antibody [EPR13424] (ab185965)

参考文献 (6)

ab185965 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185965 は 6 報の論文で使用されています。

  • Ishimura R  et al. Mechanistic insights into the roles of the UFM1 E3 ligase complex in ufmylation and ribosome-associated protein quality control. Sci Adv 9:eadh3635 (2023). PubMed: 37595036
  • Wang K  et al. The ufmylation modification of ribosomal protein L10 in the development of pancreatic adenocarcinoma. Cell Death Dis 14:350 (2023). PubMed: 37280198
  • Chen C  et al. Preparation of UFM1-Derived Probes through Highly Optimized Total Chemical Synthesis. Chemistry 29:e202300414 (2023). PubMed: 37080930
  • Ishimura R  et al. The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3. Nat Commun 13:7857 (2022). PubMed: 36543799
  • Yang R  et al. CDK5RAP3, a UFL1 substrate adaptor, is crucial for liver development. Development 146:N/A (2019). PubMed: 30635284
  • Ishimura R  et al. A novel approach to assess the ubiquitin-fold modifier 1-system in cells. FEBS Lett 591:196-204 (2017). PubMed: 27926783

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