Anti-UCP1 抗体 (ab10983)
Key features and details
- Rabbit polyclonal to UCP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-UCP1 antibody
UCP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UCP1 -
由来種
Rabbit -
特異性
This polyclonal antibody does not undergo batch testing for mouse western blot, and we cannot therefore guarantee this reagent for this species/application. For an alternative product please see ab209483 which is guaranteed for mouse western blot.
-
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Sheep, Rabbit, Cow, Dog, Human, Macaque monkey
-
免疫原
Synthetic peptide corresponding to Human UCP1 aa 145-159 conjugated to keyhole limpet haemocyanin (Cysteine residue).
-
ポジティブ･コントロール
- IHC-P: Murine inguinal white adipose tissue. WB: Rat brown fat lysate.
-
特記事項
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab10983 is affinity purified using the immunogenic peptide immobilized on agarose. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10983の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (11) |
1/1000. Detects a band of approximately 32 kDa. Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts. Staining of the UCP1 band is specifically inhibited with the immunizing peptide. This antibody may not be suitable for Western blot in Human samples.
|
| IHC-P | (8) |
1/500. This concentration is determined by indirect immunoperoxidase staining of protease digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue.
|
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 32 kDa. Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts. Staining of the UCP1 band is specifically inhibited with the immunizing peptide. This antibody may not be suitable for Western blot in Human samples. |
|
IHC-P
1/500. This concentration is determined by indirect immunoperoxidase staining of protease digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue. |
ターゲット情報
-
機能
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. -
組織特異性
Brown adipose tissue. -
配列類似性
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 281561 Cow
- Entrez Gene: 7350 Human
- Entrez Gene: 22227 Mouse
- Entrez Gene: 24860 Rat
- Omim: 113730 Human
- SwissProt: P10861 Cow
- SwissProt: P25874 Human
- SwissProt: P12242 Mouse
see all -
製品の状態
UCP1 is preferentially expressed in brown adipose tissue -
別名
- mitochondrial brown fat uncoupling protein antibody
- Mitochondrial brown fat uncoupling protein 1 antibody
- SLC25A7 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab10983)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brown adipose tissue staining UCP1 using ab10983 at 1/500 dilution. Detected using an Anti-Rabbit IgG-biotin conjugate and an Avidin-Peroxidase conjugate.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab10983)Image from Mori M et al., PLoS Biol. 2012;10(4):e1001314. Epub 2012 Apr 24. Fig 5.; doi:10.1371/journal.pbio.1001314; April 24, 2012, PLoS Biol 10(4): e1001314.ab10983, staining UCP1, in murine inguinal white adipose tissue, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was incubated with primary antibody at 1/1000 dilution and staining was detected using an HRP/DAB detection kit. Sections were counterstained using hematoxylin. -
Western blot - Anti-UCP1 antibody (ab10983)All lanes : Anti-UCP1 antibody (ab10983) at 1 µg/ml
Lane 1 : Rat brown fat
Lane 2 : Rat brown fat with UCP1 immunizing peptide (human, 145-159)
Secondary
All lanes : Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (814)
ab10983 は 814 報の論文で使用されています。
- Zhu W et al. In-frame deletion of SMC5 related with the phenotype of primordial dwarfism, chromosomal instability and insulin resistance. Clin Transl Med 13:e1007 (2023). PubMed: 36627765
- Crespo M et al. Myeloid p38 activation maintains macrophage-liver crosstalk and BAT thermogenesis through IL-12-FGF21 axis. Hepatology 77:874-887 (2023). PubMed: 35592906
- Sabaté-Pérez A et al. Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis. Autophagy 19:904-925 (2023). PubMed: 35947488
- Qian K et al. CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization. Nature 613:160-168 (2023). PubMed: 36477540
- Wang B et al. RIIβ-PKA in GABAergic Neurons of Dorsal Median Hypothalamus Governs White Adipose Browning. Adv Sci (Weinh) 10:e2205173 (2023). PubMed: 36529950