Anti-UBXN7 抗体 (ab185085)
Key features and details
- Rabbit polyclonal to UBXN7
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-UBXN7 antibody -
製品の詳細
Rabbit polyclonal to UBXN7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human UBXN7 aa 50-200.
Database link: O94888 -
ポジティブ･コントロール
- IHC-P: Human testis and skeletal muscle tissue; WB: RT4 and U-251 MG whole cell lysate; ICC: Caco-2 whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185085の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 mg/ml. Predicted molecular weight: 55 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 mg/ml. Predicted molecular weight: 55 kDa. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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配列類似性
Contains 1 UBX domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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参照データベース
- Entrez Gene: 26043 Human
- Entrez Gene: 224111 Mouse
- Entrez Gene: 303878 Rat
- SwissProt: O94888 Human
- SwissProt: Q6P5G6 Mouse
- Unigene: 518524 Human
- Unigene: 31275 Mouse
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別名
- KIAA0794 antibody
- UBX domain-containing protein 7 antibody
- UBXD7 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN7 antibody (ab185085)
Immunohistochemistry analysis of human testis tissue labeling UBXN7 (high expression) with ab185085 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-UBXN7 antibody (ab185085)All lanes : Anti-UBXN7 antibody (ab185085) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Predicted band size: 55 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-UBXN7 antibody (ab185085)Immunocytochemistry analysis of Caco-2 (human colorectal adenocarcinoma cell line) whole cells labeling UBXN7 at nucleoplasm with ab185085 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN7 antibody (ab185085)Immunohistochemistry analysis of human skeletal muscle tissue labeling UBXN7 (low expression) with ab185085 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab185085 は論文での使用が確認できていません。