Anti-UBR4/p600 抗体 (ab86738)
Key features and details
- Rabbit polyclonal to UBR4/p600
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-UBR4/p600 antibody
UBR4/p600 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UBR4/p600 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
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免疫原
Synthetic peptide corresponding to Human UBR4/p600 aa 5133-5183.
Database link: NP_065816.1 -
ポジティブ･コントロール
- WB: HeLa whole cell lysate (ab150035) IHC-P: human prostate carcinoma tissue
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特記事項
Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86738の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 574 kDa.
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| 特記事項 |
|---|
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 574 kDa. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the UBR4 family.
Contains 1 UBR-type zinc finger. -
細胞内局在
Membrane. Cytoplasm. Cytoplasm > cytoskeleton. Nucleus. Concentrates at the leading edge of membrane structures involved in actin motility. - Information by UniProt
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参照データベース
- Entrez Gene: 23352 Human
- Entrez Gene: 69116 Mouse
- Entrez Gene: 313658 Rat
- Omim: 609890 Human
- SwissProt: Q5T4S7 Human
- SwissProt: A2AN08 Mouse
- SwissProt: Q2TL32 Rat
- Unigene: 148078 Human
see all -
別名
- 600 kDa retinoblastoma protein associated factor antibody
- 600 kDa retinoblastoma protein-associated factor antibody
- E3 ubiquitin-protein ligase UBR4 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR4/p600 antibody (ab86738)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling UBR4/p600 with ab86738 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-UBR4/p600 antibody (ab86738)All lanes : Anti-UBR4/p600 antibody (ab86738) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 574 kDa
Exposure time: 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab86738 は 19 報の論文で使用されています。
- Pierga A et al. Spatacsin regulates directionality of lysosome trafficking by promoting the degradation of its partner AP5Z1. PLoS Biol 21:e3002337 (2023). PubMed: 37871017
- Varland S et al. N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity. Nat Commun 14:6774 (2023). PubMed: 37891180
- Bracci N et al. Rift Valley fever virus Gn V5-epitope tagged virus enables identification of UBR4 as a Gn interacting protein that facilitates Rift Valley fever virus production. Virology 567:65-76 (2022). PubMed: 35032865
- Niu F et al. The m6A reader YTHDF2 is a negative regulator for dendrite development and maintenance of retinal ganglion cells. Elife 11:N/A (2022). PubMed: 35179492
- Cirinelli A et al. Evidence that the transcriptional repressor ICER is regulated via the N-end rule for ubiquitination. Exp Cell Res 414:113083 (2022). PubMed: 35227662