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リコンビナントRabMAb

Anti-UBF1 抗体 [EPR2742] (ab109011)

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Western blot - Anti-UBF1 antibody [EPR2742] (ab109011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody [EPR2742] (ab109011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody [EPR2742] (ab109011)
  • Anti-UBF1 antibody [EPR2742] (ab109011)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2742] to UBF1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-UBF1 antibody [EPR2742]
    UBF1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2742] to UBF1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, A431, MCF7 and JAR cell lysates; Human colon and breast tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR2742

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109011の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 89 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 89 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.

  • 配列類似性

    Contains 6 HMG box DNA-binding domains.

  • 翻訳後修飾

    Phosphorylated and activated by PIK3CA.

  • 細胞内局在

    Nucleus > nucleolus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 90 kDa nucleolus organizer region autoantigen antibody
    • Autoantigen NOR-90 antibody
    • NOR 90 antibody
    • Nucleolar transcription factor 1 antibody
    • UBF 1 antibody
    • UBF antibody
    • UBF-1 antibody
    • UBF1_HUMAN antibody
    • Ubtf antibody
    • Upstream binding factor 1 antibody
    • upstream binding transcription factor, RNA polymerase I antibody
    • Upstream-binding factor 1 antibody
    see all

画像

  • Western blot - Anti-UBF1 antibody [EPR2742] (ab109011)
    Western blot - Anti-UBF1 antibody [EPR2742] (ab109011)
    All lanes : Anti-UBF1 antibody [EPR2742] (ab109011) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : JAR cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 89 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody [EPR2742] (ab109011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody [EPR2742] (ab109011)

    ab109011 at 1/50 dilution staining UBF1 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody [EPR2742] (ab109011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody [EPR2742] (ab109011)

    ab109011 at 1/50 dilution staining UBF1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-UBF1 antibody [EPR2742] (ab109011)
    Anti-UBF1 antibody [EPR2742] (ab109011)

参考文献 (0)

ab109011 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109011 は論文での使用が確認できていません。

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