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リコンビナントRabMAb

Anti-UBE3A 抗体 [EPR25059-12] (ab290641)

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Immunohistochemistry (Frozen sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
  • Immunohistochemistry (Frozen sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
  • Western blot - Anti-UBE3A antibody [EPR25059-12] (ab290641)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
  • Flow Cytometry (Intracellular) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
  • Flow Cytometry (Intracellular) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
  • Anti-UBE3A antibody [EPR25059-12] (ab290641)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25059-12] to UBE3A
  • Suitable for: WB, IHC-P, Flow Cyt (Intra), IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-UBE3A antibody [EPR25059-12]
    UBE3A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25059-12] to UBE3A

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, Flow Cyt (Intra), IHC-Frmore details
    適用なし: ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, HEK-293, K-562, PC-12 treated with 10 µM MG-132 for 4 hours, RAW264.7 treated with 10 µM MG-132 for 4 hours, Rat brain tissue lysate. IHC-P: Human, mouse and rat cerebrum. Flow Cyt (intra): HeLa, RAW264.7 IHC-Fr: Mouse and rat cerebrum

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290641の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 100 kDa.
IHC-P
1/500.
Flow Cyt (Intra)
1/500.
IHC-Fr
1/100.
特記事項
WB
1/1000. Predicted molecular weight: 100 kDa.
IHC-P
1/500.
Flow Cyt (Intra)
1/500.
IHC-Fr
1/100.
追加情報
Is unsuitable for ICC/IF or IP.

ターゲット情報

  • 機能

    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).

  • パスウェイ

    Protein modification; protein ubiquitination.

  • 関連疾患

    Angelman syndrome

  • 配列類似性

    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.

  • 翻訳後修飾

    Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells.

  • 細胞内局在

    Nucleus. Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ANCR antibody
      • Angelman syndrome antibody
      • AS antibody
      • CTCL tumor antigen se37 2 antibody
      • CTCL tumor antigen se37-2 antibody
      • E6 AP antibody
      • E6AP antibody
      • E6AP ubiquitin protein ligase antibody
      • E6AP ubiquitin-protein ligase antibody
      • EPVE6AP antibody
      • FLJ26981 antibody
      • HECT-type ubiquitin transferase E3A antibody
      • HPVE6A antibody
      • Human papilloma virus E6 associated protein Angelman syndrome antibody
      • Human papilloma virus E6 associated protein antibody
      • Human papillomavirus E6-associated protein antibody
      • NY REN 54 antigen antibody
      • Oncogenic protein associated protein E6 AP antibody
      • Oncogenic protein associated protein E6AP antibody
      • Oncogenic protein-associated protein E6-AP antibody
      • Renal carcinoma antigen NY REN 54 antibody
      • Renal carcinoma antigen NY-REN-54 antibody
      • UBE 3A antibody
      • Ube3a antibody
      • UBE3A protein antibody
      • UBE3A_HUMAN antibody
      • Ubiquitin protein ligase E3A antibody
      • Ubiquitin-protein ligase E3A antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
      Immunohistochemistry (Frozen sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)

      Immunohistochemical analysis of rat cerebrum (fresh) frozen sections labelling UBE3A with ab290641 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution. Nuclear staining on rat cerebrum is observed. Counter stained with DAPI.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution.

    • Immunohistochemistry (Frozen sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
      Immunohistochemistry (Frozen sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)

      Immunohistochemical analysis of mouse cerebrum (fresh) frozen sections labelling UBE3A with ab290641 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution. Nuclear staining on mouse cerebrum is observed. Counter stained with DAPI.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution.

    • Western blot - Anti-UBE3A antibody [EPR25059-12] (ab290641)
      Western blot - Anti-UBE3A antibody [EPR25059-12] (ab290641)
      All lanes : Anti-UBE3A antibody [EPR25059-12] (ab290641) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
      Lane 3 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
      Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 5 : PC-12 treated with 10 µM MG-132 for 4 hours, whole cell lysate
      Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 7 : RAW264. treated with 10 µM MG-132 for 4 hours, whole cell lysate
      Lane 8 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 100 kDa
      Observed band size: 100 kDa



      Blocking and diluting buffers: 5% NFDM/TBST. 

      Exposure time: 

      Lane 1, 2, 8: 3 minutes
      Lane 3-7: 26 seconds

      Generation of the ~50kDa degraded fragment can be inhibited/reduced by MG-132 treatment (lanes 5 and 7).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)

      Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling UBE3A with ab290641 at 1/500 dilution for 30 mins at room temerature, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining can be found in human cerebrum. 

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is preabsorbed LeicaDS9800 (BOND™ Polymer Refine Detection).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling UBE3A with ab290641 at 1/2000 dilution for 30 mins at room temerature, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining can be found in mouse cerebrum (PMID: 22787151). 

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is preabsorbed LeicaDS9800 (BOND™ Polymer Refine Detection).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody [EPR25059-12] (ab290641)

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling UBE3A with ab290641 at 1/2000 dilution for 30 mins at room temerature, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining can be found in rat cerebrum (PMID: 22787151). 

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is preabsorbed LeicaDS9800 (BOND™ Polymer Refine Detection).

    • Flow Cytometry (Intracellular) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
      Flow Cytometry (Intracellular) - Anti-UBE3A antibody [EPR25059-12] (ab290641)

      Flow cytometry analysis of 4% paraformaldehyde fixed and permeabilized with 90% methanol HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling UBE3A with ab290641 at 1/500 dilution (Red) compared with isotype control ab172730 (Black) and unlabelled control cells without incubation whith primary and secondary antibody (Blue). 

      ab150081 at 1/2000 dilution was used as secondary antibody. 

    • Flow Cytometry (Intracellular) - Anti-UBE3A antibody [EPR25059-12] (ab290641)
      Flow Cytometry (Intracellular) - Anti-UBE3A antibody [EPR25059-12] (ab290641)

      Flow cytometry analysis of 4% paraformaldehyde fixed and permeabilized with 90% methanol RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling UBE3A with ab290641 at 1/500 dilution (Red) compared with isotype control ab172730 (Black) and unlabelled control cells without incubation whith primary and secondary antibody (Blue). 

      ab150081 at 1/2000 dilution was used as secondary antibody. 

    • Anti-UBE3A antibody [EPR25059-12] (ab290641)
      Anti-UBE3A antibody [EPR25059-12] (ab290641)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab290641 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290641 は論文での使用が確認できていません。

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