Anti-Ube2N / Ubc13 抗体 (ab25885)
Key features and details
- Rabbit polyclonal to Ube2N / Ubc13
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Ube2N / Ubc13 antibody
Ube2N / Ubc13 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ube2N / Ubc13 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- WB: Human small intestine cell lysate. ICC/IF: Hep G2 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25885の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 18 kDa.
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 18 kDa. |
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
-
機能
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the ubiquitin-conjugating enzyme family. -
翻訳後修飾
Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 7334 Human
- Entrez Gene: 93765 Mouse
- Entrez Gene: 116725 Rat
- Omim: 603679 Human
- SwissProt: P61088 Human
- SwissProt: P61089 Mouse
- SwissProt: Q9EQX9 Rat
- Unigene: 524630 Human
see all -
別名
- Bendless like ubiquitin conjugating enzyme antibody
- Bendless-like ubiquitin-conjugating enzyme antibody
- BLU antibody
see all
画像
-
Western blot - Anti-Ube2N / Ubc13 antibody (ab25885)Lane 1 : Anti-Ube2N / Ubc13 antibody (ab25885) at 0.5 µg/ml
Lane 2 : Anti-Ube2N / Ubc13 antibody (ab25885) at 1 µg/ml
Lane 3 : Anti-Ube2N / Ubc13 antibody (ab25885) at 2 µg/ml
All lanes : Human small intestine cell lysates
Lysates/proteins at 15 µg per lane.
Predicted band size: 18 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2N / Ubc13 antibody (ab25885)ab25885 at 2µg/ml staining UBe2N in mouse thymus tissue by IHC
-
Immunocytochemistry/ Immunofluorescence - Anti-Ube2N / Ubc13 antibody (ab25885)ICC/IF image of ab25885 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25885, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (5)
ab25885 は 5 報の論文で使用されています。
- Wenger A et al. CRISPR-Cas9 knockout screen identifies novel treatment targets in childhood high-grade glioma. Clin Epigenetics 15:80 (2023). PubMed: 37161535
- Zhang X et al. The inhibition of UBC13 expression and blockage of the DNMT1-CHFR-Aurora A pathway contribute to paclitaxel resistance in ovarian cancer. Cell Death Dis 9:93 (2018). PubMed: 29367628
- Maculins T et al. A Generic Platform for Cellular Screening Against Ubiquitin Ligases. Sci Rep 6:18940 (2016). PubMed: 26743172
- Nakada S et al. RNF8 regulates assembly of RAD51 at DNA double-strand breaks in the absence of BRCA1 and 53BP1. Cancer Res 72:4974-83 (2012). PubMed: 22865450
- Kocevar N et al. Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers. World J Gastroenterol 18:1216-28 (2012). WB ; Human . PubMed: 22468085