Anti-UBE2I / UBC9 抗体 (ab33044)
Key features and details
- Rabbit polyclonal to UBE2I / UBC9
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-UBE2I / UBC9 antibody
UBE2I / UBC9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UBE2I / UBC9 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Xenopus laevis, Zebrafish
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Recombinant Human UBE2I / UBC9 protein (ab127405) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HeLa, Jurkat, A431, NIH3T3, MEF1, PC12, This antibody gave a positive signal in the following tissue lysate:Testis (Mouse) Tissue Lysate - normal tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33044の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| IP |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. -
組織特異性
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. -
パスウェイ
Protein modification; protein sumoylation. -
配列類似性
Belongs to the ubiquitin-conjugating enzyme family. -
細胞内局在
Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body. - Information by UniProt
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参照データベース
- Entrez Gene: 374123 Chicken
- Entrez Gene: 7329 Human
- Entrez Gene: 100044900 Mouse
- Entrez Gene: 22196 Mouse
- Entrez Gene: 25573 Rat
- Entrez Gene: 380450 Xenopus laevis
- Entrez Gene: 114445 Zebrafish
- Omim: 601661 Human
see all -
別名
- C358B7.1 antibody
- p18 antibody
- SUMO 1 protein ligase antibody
see all
画像
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Western blot - Anti-UBE2I / UBC9 antibody (ab33044)All lanes : Anti-UBE2I / UBC9 antibody (ab33044) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 : A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa -
Western blot - Anti-UBE2I / UBC9 antibody (ab33044)All lanes : Anti-UBE2I / UBC9 antibody (ab33044) at 1 µg/ml
Lane 1 : NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-UBE2I / UBC9 antibody (ab33044)UBE2I / UBC9 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to UBE2I / UBC9 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33044.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 18kDa: UBE2I / UBC9. -
Immunocytochemistry/ Immunofluorescence - Anti-UBE2I / UBC9 antibody (ab33044)ICC/IF image of ab33044 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33044, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2I / UBC9 antibody (ab33044)IHC image of ab33044 staining UBE2I / UBC9 in Human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33044, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab33044 は 10 報の論文で使用されています。
- Ma Y et al. The DACH1 Gene Transcriptional Activation and Protein Degradation Mediated by Transactivator Tas of Prototype Foamy Virus. Viruses 15:N/A (2023). PubMed: 37766305
- Wang T et al. SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism. Mol Cell 75:823-834.e5 (2019). PubMed: 31302001
- Colnaghi L et al. Super Resolution Microscopy of SUMO Proteins in Neurons. Front Cell Neurosci 13:486 (2019). PubMed: 31749687
- Shastrula PK et al. PML is recruited to heterochromatin during S phase and represses DAXX-mediated histone H3.3 chromatin assembly. J Cell Sci 132:N/A (2019). PubMed: 30796101
- Rodriguez A et al. Loss of the E2 SUMO-conjugating enzyme Ube2i in oocytes during ovarian folliculogenesis causes infertility in mice. Development 146:N/A (2019). PubMed: 31704792
- Bogachek MV et al. Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas. Stem Cell Reports 7:1140-1151 (2016). PubMed: 27916539
- Datta S et al. A pathway linking oxidative stress and the Ran GTPase system in progeria. Mol Biol Cell 25:1202-15 (2014). WB . PubMed: 24523287
- Kelley JB et al. The defective nuclear lamina in hutchinson-gilford progeria syndrome disrupts the nucleocytoplasmic ran gradient and inhibits nuclear localization of ubc9. Mol Cell Biol 31:3378-95 (2011). PubMed: 21670151
- Herkert B et al. The Arf tumor suppressor protein inhibits Miz1 to suppress cell adhesion and induce apoptosis. J Cell Biol 188:905-18 (2010). WB, ICC/IF ; Human . PubMed: 20308430
- Mimura Y et al. Two-step colocalization of MORC3 with PML nuclear bodies. J Cell Sci 123:2014-24 (2010). WB ; Mouse . PubMed: 20501696