Anti-UBE2I / UBC9 抗体 (ab21193)
Key features and details
- Goat polyclonal to UBE2I / UBC9
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-UBE2I / UBC9 antibody
UBE2I / UBC9 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to UBE2I / UBC9 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Chicken, Hamster, Dog, Xenopus laevis
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免疫原
Synthetic peptide:
SGIALSRLAQERK
, corresponding to N terminal amino acids 2-14 of Human Ube2I. -
特記事項
Product is very sensitive to freeze/thawing therefore this should be avoided. Store at 4C for short term use and -20C for long term storage in small aliquots.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21193の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| WB | (1) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa).
A 1 hour primary incubation is recommended for this product. Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature. |
| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa). A 1 hour primary incubation is recommended for this product. Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature. |
ターゲット情報
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機能
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. -
組織特異性
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. -
パスウェイ
Protein modification; protein sumoylation. -
配列類似性
Belongs to the ubiquitin-conjugating enzyme family. -
細胞内局在
Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body. - Information by UniProt
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参照データベース
- Entrez Gene: 374123 Chicken
- Entrez Gene: 491332 Dog
- Entrez Gene: 7329 Human
- Entrez Gene: 25573 Rat
- Entrez Gene: 380450 Xenopus laevis
- Omim: 601661 Human
- SwissProt: P63283 Chicken
- SwissProt: P63279 Human
see all -
別名
- C358B7.1 antibody
- p18 antibody
- SUMO 1 protein ligase antibody
see all
画像
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Western blot - Anti-UBE2I / UBC9 antibody (ab21193)Anti-UBE2I / UBC9 antibody (ab21193) at 0.3 µg/ml + Rat ovary lysate at 35 µg
Predicted band size: 18 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2I / UBC9 antibody (ab21193)Ab21193 (0.3ug/ml) staining human UBE2I in human kidney by immunohistochemistry using paraffin embedded tissue.Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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Western blot - Anti-UBE2I / UBC9 antibody (ab21193)Anti-UBE2I / UBC9 antibody (ab21193) at 0.1 µg/ml + Human kidney lysate at 35 µg
Predicted band size: 18 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab21193 は 18 報の論文で使用されています。
- Yatskevich S et al. Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation. Cell Rep 34:108929 (2021). PubMed: 33789095
- Guérillon C et al. Multisite SUMOylation restrains DNA polymerase ? interactions with DNA damage sites. J Biol Chem 295:8350-8362 (2020). PubMed: 32350109
- Johnson LM et al. Short-term heritable variation overwhelms 200 generations of mutational variance for metabolic traits in Caenorhabditis elegans. Evolution 74:2451-2464 (2020). PubMed: 32989734
- Xu J et al. A Provocative Molecular Link between Mammographic Density and BRCA1-loss associated TNBC. Int J Hum Genet Genet Disord 1:1-8 (2019). PubMed: 33860286
- Heras G et al. Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification. J Mol Cell Biol 11:356-370 (2019). PubMed: 29868881