Anti-UBE2E2 抗体 [EPR13003(B)] (ab177485)
![Western blot - Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)](https://www.abcam.co.jp/ps/products/177/ab177485/Images/ab177485-205412-anti-ube2e2-antibody-epr13003b-western-blot.jpg "Western blot - Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13003(B)] to UBE2E2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-UBE2E2 antibody [EPR13003(B)]
UBE2E2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13003(B)] to UBE2E2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human UBE2E2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96LR5 -
ポジティブ･コントロール
- HeLa, LNCaP, 293T and Jurkat whole cell lysate (ab7899).
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR13003(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177485の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
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| 特記事項 |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa). |
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.
ターゲット情報
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機能
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the ubiquitin-conjugating enzyme family. - Information by UniProt
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参照データベース
- Entrez Gene: 7325 Human
- Entrez Gene: 218793 Mouse
- Entrez Gene: 361013 Rat
- Omim: 602163 Human
- SwissProt: Q96LR5 Human
- SwissProt: Q91W82 Mouse
- Unigene: 475688 Human
- Unigene: 595802 Human
see all -
別名
- FLJ25157 antibody
- UB2E2_HUMAN antibody
- UBC4/5 homolog yeast antibody
see all
画像
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Western blot - Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)All lanes : Anti-UBE2E2 antibody [EPR13003(B)] (ab177485) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : LNCaP cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa -
![Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)](https://www.abcam.co.jp/ps/products/177/ab177485/Images/ab177485-2-benefits-of-recombinant-antibodies.png)
Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab177485 は 1 報の論文で使用されています。
- Gan N et al. Legionella pneumophila inhibits immune signalling via MavC-mediated transglutaminase-induced ubiquitination of UBE2N. Nat Microbiol 4:134-143 (2019). PubMed: 30420781